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| Status |
Public on Apr 27, 2023 |
| Title |
Comparative transcriptomic anlaysis of postharvest stored ‘Bingtangcheng’ and ‘Tarocco’ Blood orange fruit differing in fruit citrate and anthocyanin accumulation |
| Organism |
Citrus sinensis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Organic acids and anthocyanins are the most important compounds for the flavor and nutritional quality of citrus fruit. In this study, a comparative transcriptome analysis was conducted to elucidate the genes and pathways involved in the accumulation of citrate and anthocyanins in postharvest citrus fruit using ‘Tarocco’ blood orange (TBO) and ‘Bingtangcheng’ sweet orange (BTSO). About 2397, 2677, 3067, 3131 and 2960 differentially expressed genes (DEGs) between two materials were identified at 0, 15, 30, 60, and 90 DAS (days after storage) respectively. A robust core set of 825 DEGs temporally associated with the citrate and anthocyanin accumulation throughout the storage period. Further function enrichment revealed the up-regulated DEGs in TBO were mainly related to UV protection, flavonoid biosynthesis process and transcription factors (TFs). And the down-regulated DEGs were mainly related to inositol biosynthesis process, carboxylic acid/organic acid transmembrane transport and transporters. Moreover, co-expression network and correlation analysis revealed that 23 differentially expressed TFs may be responsible for the simultaneously positively regulation of citrate and anthocyanin accumulation. And 19 differentially expressed transporters may be involved in citrate metabolism and negatively correlated with citrate content. These results suggesting that the high anthocyanis and citrate levels in TBO compared with BTSO were porbably attributed to the highly active of a group of core TFs and lowly active of transporters, which will accordingly provide novel insights into the molecular mechanisms underlying the difference accumulation of citrate and anthocyanin content in TBO and BTSO.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for postharvest ‘Tarocco’ Blood orange and ‘Bingtangcheng' sweet orange fruit.
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| Contributor(s) |
Jin Y, Liao M, Li N, Ma X, Zhang H, Han J, Li D, Yang J, Lu X, Sheng L |
| Citation(s) |
37268922 |
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| Submission date |
Aug 25, 2022 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Ling Sheng |
| E-mail(s) |
shengling0629@hunau.edu.cn
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| Organization name |
Hunan Agricultural University
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| Street address |
Donghu
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| City |
Changsha |
| ZIP/Postal code |
410128 |
| Country |
China |
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| Platforms (1) |
| GPL26104 |
Illumina HiSeq 4000 (Citrus sinensis) |
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| Samples (30)
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| GSM6507381 |
‘Bingtangcheng' fruit juice sac, 0 days after storege, Biol rep 1 |
| GSM6507382 |
‘Bingtangcheng' fruit juice sac, 0 days after storege, Biol rep 2 |
| GSM6507383 |
‘Bingtangcheng' fruit juice sac, 0 days after storege, Biol rep 3 |
| GSM6507384 |
‘Tarocco’ Blood orange juice sac, 0 days after storege, Biol rep 1 |
| GSM6507385 |
‘Tarocco’ Blood orange juice sac, 0 days after storege, Biol rep 2 |
| GSM6507386 |
‘Tarocco’ Blood orange juice sac, 0 days after storege, Biol rep 3 |
| GSM6507387 |
‘Bingtangcheng' fruit juice sac, 15 days after storege, Biol rep 1 |
| GSM6507388 |
‘Bingtangcheng' fruit juice sac, 15 days after storege, Biol rep 2 |
| GSM6507389 |
‘Bingtangcheng' fruit juice sac, 15 days after storege, Biol rep 3 |
| GSM6507390 |
‘Tarocco’ Blood orange juice sac, 15 days after storege, Biol rep 1 |
| GSM6507391 |
‘Tarocco’ Blood orange juice sac, 15 days after storege, Biol rep 2 |
| GSM6507392 |
‘Tarocco’ Blood orange juice sac, 15 days after storege, Biol rep 3 |
| GSM6507393 |
‘Bingtangcheng' fruit juice sac, 30 days after storege, Biol rep 1 |
| GSM6507394 |
‘Bingtangcheng' fruit juice sac, 30 days after storege, Biol rep 2 |
| GSM6507395 |
‘Bingtangcheng' fruit juice sac, 30 days after storege, Biol rep 3 |
| GSM6507396 |
‘Tarocco’ Blood orange juice sac, 30 days after storege, Biol rep 1 |
| GSM6507397 |
‘Tarocco’ Blood orange juice sac, 30 days after storege, Biol rep 2 |
| GSM6507398 |
‘Tarocco’ Blood orange juice sac, 30 days after storege, Biol rep 3 |
| GSM6507399 |
‘Bingtangcheng' fruit juice sac, 60 days after storege, Biol rep 1 |
| GSM6507400 |
‘Bingtangcheng' fruit juice sac, 60 days after storege, Biol rep 2 |
| GSM6507401 |
‘Bingtangcheng' fruit juice sac, 60 days after storege, Biol rep 3 |
| GSM6507402 |
‘Tarocco’ Blood orange juice sac, 60 days after storege, Biol rep 1 |
| GSM6507403 |
‘Tarocco’ Blood orange juice sac, 60 days after storege, Biol rep 2 |
| GSM6507404 |
‘Tarocco’ Blood orange juice sac, 60 days after storege, Biol rep 3 |
| GSM6507405 |
‘Bingtangcheng' fruit juice sac, 90 days after storege, Biol rep 1 |
| GSM6507406 |
‘Bingtangcheng' fruit juice sac, 90 days after storege, Biol rep 2 |
| GSM6507407 |
‘Bingtangcheng' fruit juice sac, 90 days after storege, Biol rep 3 |
| GSM6507408 |
‘Tarocco’ Blood orange juice sac, 90 days after storege, Biol rep 1 |
| GSM6507409 |
‘Tarocco’ Blood orange juice sac, 90 days after storege, Biol rep 2 |
| GSM6507410 |
‘Tarocco’ Blood orange juice sac, 90 days after storege, Biol rep 3 |
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| Relations |
| BioProject |
PRJNA873284 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212015_All_genes_fpkm_expression.txt.gz |
6.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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