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| Status |
Public on Aug 21, 2022 |
| Title |
RNA-Seq of Hrip1-treated rice leaves |
| Organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hrip1 was isolated from Alternaria tenuissima and was found to induce systematic acquired resistance in tobacco. In rice plants, it was found to confer immunity against rice blast fungi. To understand the genomic signature of Hrip1-induced immunity, rice seedlings were treated with 30 nm Hrip1 protein and triplicate leaf samples were subjected to sequencing on the BIGSEQ500 platform.
Two groups of rice seedlings were grown to the three-leaf stage. The experiment group was treated with 30nm Hrip1 protein while the control plants were treated with Tris-HCl buffer. Triplicate samples from both setups were taken at 6, 12, 24, and 48 hr after treatment. Total RNA was extracted and reverse transcribed in first strand cDNA as input material.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for Hrip1-treated samples (HT) and control samples (CB) (Treatment versus control).
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| Contributor(s) |
Ninkuu V, Yan J, Zhang L, Fu Z, Yang T, Li S, Li B, Duan J, Ren J, Li G, Yang X, Zeng H |
| Citation(s) |
36212323 |
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| Submission date |
Aug 17, 2022 |
| Last update date |
Oct 27, 2022 |
| Contact name |
Zeng Hongmei |
| E-mail(s) |
zenghongmei@caas.cn
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Biological Control
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| Lab |
Protein Pesticides
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| Street address |
Chinese Academy of Agricultural Sciences, 12 Zhongguancun,, Haidian district, Building 39, International Students Dorm
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
+86 |
| Country |
China |
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| Platforms (1) |
| GPL27908 |
BGISEQ-500 (Oryza sativa Japonica Group) |
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| Samples (24)
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| GSM6475016 |
CB62 Control leaf,6 hr |
| GSM6475017 |
HT63 leaf, 6 hr |
| GSM6475018 |
CB63 Control leaf,6 hr |
| GSM6475019 |
HT121 leaf, 12 hr |
| GSM6475020 |
CB121 Control leaf,12 hr |
| GSM6475021 |
HT122 leaf, 12 hr |
| GSM6475022 |
CB122 Control leaf,12 hr |
| GSM6475023 |
HT123 leaf, 12 hr |
| GSM6475024 |
CB123 Control leaf,12 hr |
| GSM6475025 |
HT241 leaf, 24 hr |
| GSM6475026 |
CB241 Control leaf,24 hr |
| GSM6475027 |
HT242 leaf, 24 hr |
| GSM6475028 |
CB242 Control leaf,24 hr |
| GSM6475029 |
HT243 leaf, 24 hr |
| GSM6475030 |
CB243 Control leaf,24 hr |
| GSM6475031 |
HT481 leaf, 48 hr |
| GSM6475032 |
CB481 Control leaf,48 hr |
| GSM6475033 |
HT482 leaf, 48 hr |
| GSM6475034 |
CB482 Control leaf,48 hr |
| GSM6475035 |
HT483 leaf, 48 hr |
| GSM6475036 |
CB483 Control leaf,48 hr |
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| Relations |
| BioProject |
PRJNA870590 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211516_CB12-VS-HT12.GeneDiffExp.txt.gz |
3.7 Mb |
(ftp)(http) |
TXT |
| GSE211516_CB12-VS-HT12.GeneDiffExpFilter.txt.gz |
45.1 Kb |
(ftp)(http) |
TXT |
| GSE211516_CB24-VS-HT24.GeneDiffExp.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| GSE211516_CB24-VS-HT24.GeneDiffExpFilter.txt.gz |
47.6 Kb |
(ftp)(http) |
TXT |
| GSE211516_CB48-VS-HT48.GeneDiffExp.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| GSE211516_CB48-VS-HT48.GeneDiffExpFilter.txt.gz |
115.9 Kb |
(ftp)(http) |
TXT |
| GSE211516_CB6-VS-HT6.GeneDiffExp.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE211516_CB6-VS-HT6.GeneDiffExpFilter.txt.gz |
46.6 Kb |
(ftp)(http) |
TXT |
| GSE211516_RAW.tar |
9.5 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE211516_all.gene.FPKM.txt.gz |
4.6 Mb |
(ftp)(http) |
TXT |
| Raw data not provided for this record |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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