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| Status |
Public on Aug 11, 2022 |
| Title |
A twin UGUA motif directs the balance between gene isoforms through CFIm and the mTORC1 signaling pathway |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
To identify APA changes in Tsc1-cKO mice, cTag-PAPERCLIP was performed on pAAV-Camk2a-iCre injected Tsc1-WT and Tsc1-floxed cTag-PABP mice. To identify CPSF6-dependent PAS, PAPERCLIP was performed on shCPSF6-BE2C cells with/without doxycycline treatment.
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| Overall design |
cTag-PAPERCLIP and PAPERCLIP library construction was performed as previously described (Hwang et al., Neuron 2017; Kunisky et al., Cell Rep 2021) and the libraries were sequenced on Illumina NextSeq
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| Contributor(s) |
Herron R, Kunisky AK, Madden JR, Anyaeche VI, Maung MZ, Hwang H |
| Citation(s) |
37665675 |
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| Submission date |
Aug 08, 2022 |
| Last update date |
Sep 05, 2023 |
| Contact name |
Hun-Way Hwang |
| E-mail(s) |
Hunway.Hwang@pitt.edu
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| Organization name |
University of Pittsburgh
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| Department |
Department of Pathology
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| Lab |
S754 Scaife Hall
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| Street address |
3550 Terrace Street
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| GSM6436856 |
Tsc1-WT, cTag-PABP, pAAV-Camk2a-iCre, Rep1 |
| GSM6436857 |
Tsc1-KO, cTag-PABP, pAAV-Camk2a-iCre, Rep1 |
| GSM6436858 |
Tsc1-WT, cTag-PABP, pAAV-Camk2a-iCre, Rep2 |
| GSM6436859 |
Tsc1-KO, cTag-PABP, pAAV-Camk2a-iCre, Rep2 |
| GSM6436860 |
shCPSF6-BE2C, NoDox, Rep1 |
| GSM6436861 |
shCPSF6-BE2C, PlusDox, Rep1 |
| GSM6436862 |
shCPSF6-BE2C, NoDox, Rep2 |
| GSM6436863 |
shCPSF6-BE2C, PlusDox, Rep2 |
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| Relations |
| BioProject |
PRJNA867395 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE210768_RAW.tar |
710.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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