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| Status |
Public on Sep 09, 2022 |
| Title |
A ribosomal protein homolog modulates gene expression and virulence in a bacterial pathogen |
| Organism |
Francisella tularensis subsp. holarctica LVS |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To assess which F. tularensis LVS transcripts are impacted by loss of bS21-2, we generated cells lacking the gene encoding bS21-2 (LVS ∆rpsU2). We then grew wild-type cells containing an empty vector (LVS pF), cells lacking bS21-2 with empty vector (LVS ∆rpsU2 pF), and cells lacking the native bS21-2 but ectopically expressing bS21-2 from a plasmid (LVS ∆rpsU2 pF-rpsU2-V). We isolated RNA from mid-log phase cells and analyzed by RNA-Seq.
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| Overall design |
Comparative gene expression profiling analysis of RNA-Seq data for wild-type F. tularensis LVS with empty vector (WT pF), cells lacking bS21-2 with empty vector (LVS ∆rpsU2 pF), and LVS ∆rpsU2 ectopically expressing bS21-2 (LVS ∆rpsU2 pF-rpsU2-V)
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| |
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| Contributor(s) |
Trautmann H, Ramsey KM |
| Citation(s) |
36121290 |
| |
| Submission date |
Aug 08, 2022 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Kathryn M Ramsey |
| E-mail(s) |
kramsey@uri.edu
|
| Organization name |
University of Rhode Island
|
| Department |
CMB / BPS
|
| Lab |
Ramsey Lab
|
| Street address |
7 Greenhouse Road
|
| City |
Kingston |
| State/province |
RI |
| ZIP/Postal code |
02881 |
| Country |
USA |
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| Platforms (1) |
| GPL32555 |
NextSeq 2000 (Francisella tularensis subsp. holarctica LVS) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA867393 |
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