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Series GSE21070 Query DataSets for GSE21070
Status Public on Sep 20, 2010
Title Expression profile of contrasting maize genotypes grown on acid and control soil (root tips)
Organism Zea mays
Experiment type Expression profiling by array
Summary Aluminum toxicity is one of the major limiting factors for many crops worldwide. The primary symptom of Al toxicity syndrome is the inhibition of root growth, leading to poor water and nutrient absorption. The causes of this inhibition are still elusive, with several biochemical pathways being affected and with a significant variation between species. Most of the work done so far to investigate the genes responsible for Al tolerance used hydroponic culture. Here we evaluated plant responses using soil as substrate, which is a condition closer to the field reality.
We used Affymetrix chips to reveal the transcriptional changes of two maize genotypes contrasting for Al tolerance.
 
Overall design Root tips from Cat100-6 (Al-tolerant) and S1587-17 (Al-sensitive) maize genotypes were harvested after growing on acid or control soil for 1 or 3 days. Total RNA was extracted and hybridized to Affymetrix GeneChip Maize Genome Array. Three biological replicates were used for each sample.
 
Contributor(s) Mattiello L, Menossi M, Kirst M, Jorge RA
Citation(s) 20828383
Submission date Mar 25, 2010
Last update date Mar 22, 2012
Contact name Marcelo Menossi
E-mail(s) menossi@unicamp.br
Phone 551935216236
Fax 551935216241
Organization name Marcelo Menossi
Department Genetica, Evolucao e Bioagentes
Lab Laboratorio de Genoma Funcional
Street address Rua Monteiro Lobato, 255, Instituto de Biologia, Departamento de Genetica, Evolucao e Bioagentes
City Campinas
State/province SP
ZIP/Postal code 13083-970
Country Brazil
 
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (24)
GSM526793 Cat100-6, control soil, 1 day treatment, biologial rep1
GSM526794 Cat100-6, acid soil, 1 day treatment, biologial rep1
GSM526795 Cat100-6, control soil, 1 day treatment, biologial rep2
Relations
BioProject PRJNA126735

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21070_RAW.tar 48.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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