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| Status |
Public on Jul 31, 2024 |
| Title |
Regulation of blood-brain barrier by astrocyte through mitochondrial transfer. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous data revealed the role of Mfn2 in mediating mitochondrial transfer from a subset of astrocytes that express Dmp1 to cerebrovascular endothelial cells. We used scRNA-seq to investigate the role of Mfn2-mediated mitochondrial transfer in endothelial cell functions.
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| Overall design |
Mfn2 was conditionally depleted in astrocytesDMP1 by establishing Dmp1Cre-Mfn2flox/flox transgenic mice with Dmp1Cre and Mfn2flox/flox mouse lineages. Next, 3 wild type (WT) mouse brains and 3 age-matched Dmp1Cre-Mfn2flox/flox mouse brains were digested into single cells by standard protocols and mixed into one sample, respectively. Mixed single cells from WT mouse brains and Dmp1Cre-Mfn2flox/flox mouse brains were processed for further scRNA sequencing.
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| Contributor(s) |
Gao J, Liu D |
| Citation(s) |
38941455 |
| |
| Submission date |
Aug 01, 2022 |
| Last update date |
Aug 01, 2024 |
| Contact name |
Junjie Gao |
| E-mail(s) |
Junjie.gao@shsmu.edu.cn
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| Phone |
+8615067175608
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| Organization name |
Shanghai Jiao Tong University Affiliated Shanghai Sixth People’s Hospital
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| Street address |
600 Yishan Road
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| City |
Shanghai |
| ZIP/Postal code |
200233 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM6423085 |
WT-Brains, replicate 1, scRNA-seq |
| GSM6423086 |
Dmp1Cre-Mfn2flox/flox-Brains, replicate 1, scRNA-seq |
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| Relations |
| BioProject |
PRJNA864662 |
