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| Status |
Public on Jun 22, 2010 |
| Title |
Genome wide FOXP3 binding sites in human cord blood derived CD4+CD25+ natural T regulatory cells (nTreg) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
FOXP3 is essential for the stability and function of nTReg. We have characterised the FOXP3-dependent cis-regulatory network through a combination of whole genome ChIP-on-chip and transcriptional profiling of primary human nTreg cells . Human nTreg cells were isolated from cord blood and expanded ex vivo (day9). These analyses identified approximately 8,000 high quality FOXP3 binding sites the majority (85%) of which were located in proximity to annotated genes . Intersection of DNA binding data with gene expression profiles predicted 739 candidate target genes.
The supplementary bed file contains all 8305 high quality binding FOXP3 binding regions reported in the paper.
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| |
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| Overall design |
Day9 expanded cord blood nTreg cells ChIPed with polyclonal rabbit anti-Foxp3 IgG (Novus Biochem) vs. Input chromatin
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| |
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| Contributor(s) |
Sadlon TJ, Pederson SM, Barry SC |
| Citation(s) |
20554955, 35773720 |
| |
| Submission date |
Mar 22, 2010 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Stephen Martin Pederson |
| E-mail(s) |
stephen.pederson@adelaide.edu.au
|
| Phone |
+614 1333 9618
|
| Organization name |
University of Adelaide
|
| Department |
Biological Sciences
|
| Lab |
Boinformatics Hub
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5005 |
| Country |
Australia |
| |
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| Platforms (7)
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| GPL4910 |
[Hs35b_P01R] Affymetrix Human Tiling 2.0R Set, Array 1 |
| GPL4911 |
[Hs35b_P02R] Affymetrix Human Tiling 2.0R Set, Array 2 |
| GPL4912 |
[Hs35b_P03R] Affymetrix Human Tiling 2.0R Set, Array 3 |
| GPL4913 |
[Hs35b_P04R] Affymetrix Human Tiling 2.0R Set, Array 4 |
| GPL4914 |
[Hs35b_P05R] Affymetrix Human Tiling 2.0R Set, Array 5 |
| GPL4915 |
[Hs35b_P06R] Affymetrix Human Tiling 2.0R Set, Array 6 |
| GPL4916 |
[Hs35b_P07R] Affymetrix Human Tiling 2.0R Set, Array 7 |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA124511 |
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