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| Status |
Public on Oct 31, 2023 |
| Title |
Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the alterations that occur in the TGF-B signalling pathway in Alström syndrome, we established by lentiviral transduction with CRISPR an hTERT-BJ-5ta cell line with the ALMS1 gene inhibited.
We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells (WT and KO) after 24h of TGF-β stimulation.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for hTERT BJ-5ta cells and its KO derivatives for ALMS1 gene (ALMS1KO) after TGF-β stimulation.
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| Contributor(s) |
Bea-Mascato B |
| Citation(s) |
38062477 |
| |
| Submission date |
Jul 27, 2022 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Brais Bea Mascato |
| Organization name |
University of Vigo
|
| Street address |
Campus de Vigo As LagoasCtra. de Marcosende, 36310 Vigo, Pontevedra
|
| City |
Vigo |
| State/province |
Pontevedra |
| ZIP/Postal code |
36310 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (6)
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| GSM6402410 |
hTERT-BJ-5ta cells, ALMS1 Knockout (KO) 1 |
| GSM6402411 |
hTERT-BJ-5ta cells, ALMS1 Knockout (KO) 2 |
| GSM6402412 |
hTERT-BJ-5ta cells, ALMS1 Knockout (KO) 3 |
|
| Relations |
| BioProject |
PRJNA862693 |
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