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Series GSE209844 Query DataSets for GSE209844
Status Public on Oct 31, 2023
Title Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the alterations that occur in the TGF-B signalling pathway in Alström syndrome, we established by lentiviral transduction with CRISPR an hTERT-BJ-5ta cell line with the ALMS1 gene inhibited.
We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells (WT and KO) after 24h of TGF-β stimulation.
Overall design Comparative gene expression profiling analysis of RNA-seq data for hTERT BJ-5ta cells and its KO derivatives for ALMS1 gene (ALMS1KO) after TGF-β stimulation.
Contributor(s) Bea-Mascato B
Citation(s) 38062477
Submission date Jul 27, 2022
Last update date Dec 14, 2023
Contact name Brais Bea Mascato
Organization name University of Vigo
Street address Campus de Vigo As LagoasCtra. de Marcosende, 36310 Vigo, Pontevedra
City Vigo
State/province Pontevedra
ZIP/Postal code 36310
Country Spain
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (6)
GSM6402407 hTERT-BJ-5ta cells, Wild type (WT) 1
GSM6402408 hTERT-BJ-5ta cells, Wild type (WT) 2
GSM6402409 hTERT-BJ-5ta cells, Wild type (WT) 3
BioProject PRJNA862693

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE209844_CountMatrixMerged.txt.gz 389.3 Kb (ftp)(http) TXT
GSE209844_NormalizedCountMatrix_DESeq2.txt.gz 891.1 Kb (ftp)(http) TXT
GSE209844_NormalizedCountMatrix_rlog.txt.gz 1.4 Mb (ftp)(http) TXT
GSE209844_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
GSE209844_RNA-seq_results_DESeq2.txt.gz 1.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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