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| Status |
Public on Oct 25, 2022 |
| Title |
The extraretinal photoreceptor OPSIN3 in the hypothalamus is involved in the regulation of water balance, body temperature and motor activity |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the role of the light-sensitive protein OPSIN3 in the supraoptic nucleus (SON) of the hypothalamus, we knocked down the gene encoding this protein (Opn3) in one SON of sprague dawley rats by delivering a Opn3 short hairpin RNA (shRNA). A non-targeting ShRNA was delivered to the opppsite SON in the same rat as a contros.We then performed gene expression profiling analysis using data obtained from RNA-seq of 5 different rats at one time point.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for Opn3 knock down SON samples in comparison to control SON samples.
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| Contributor(s) |
Bárez-López S, Elsamad G, Bishop P, Murphy D, Greenwood MP |
| Citation missing |
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| |
| Submission date |
Jul 22, 2022 |
| Last update date |
Oct 25, 2022 |
| Contact name |
Ghadir ElSamad |
| E-mail(s) |
jn18641@bristol.ac.uk
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| Phone |
07767591961
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| Organization name |
University of Bristol
|
| Street address |
Whitson St.
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| City |
Bristol |
| ZIP/Postal code |
BS1 3NY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA861255 |
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