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| Status |
Public on Mar 16, 2010 |
| Title |
Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Many studies of cocaine-responsive gene expression have focused on changes occurring during cocaine exposure, but few studies have examined the persistence of these changes with cocaine abstinence. Persistent changes in gene expression, as well as alterations induced during abstinence may underlie long-lasting drug craving and relapse liability.
Results: Whole-genome expression analysis was conducted on a rat cocaine binge-abstinence model that has previously been demonstrated to engender increased drug seeking and taking with abstinence. Gene expression changes in two mesolimbic terminal fields (mPFC and NAc) were identified in a comparison of cocaine-naïve rats with rats after 10 days of cocaine selfadministration followed by 1, 10, or 100 days of enforced abstinence (n=6-11 per group). A total of 1,461 genes in the mPFC and 414 genes in the NAc were altered between at least two time points (ANOVA, p<0.05; ±1.4 fold-change). These genes can be subdivided into: 1) changes with cocaine self-administration that do not persist into periods of abstinence, 2) changes with cocaine self-administration that persist with abstinence, 3) and those not changed with cocaine self-administration, but changed during enforced abstinence. qPCR analysis was conducted to confirm gene expression changes observed in the microarray analysis.
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| Overall design |
Gene expression changes in two mesolimbic terminal fields (mPFC and Nac) were identified in a comparison of cocaine-naϊve rats with rats after 10 days of cocaine self-administration followed by 1, 10 or 100 days of enforced abstinence. 5-6 replicates per condition.
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| Contributor(s) |
Freeman WM, Lull ME, Patel KM, Brucklacher RM, Morgan D, Roberts DC, Vrana KE |
| Citation(s) |
20187946 |
| Submission date |
Mar 15, 2010 |
| Last update date |
Jun 06, 2014 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
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| Department |
Genome Sciences Facility
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| Street address |
500 University Drive
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL2896 |
GE Healthcare/Amersham Biosciences CodeLink™ Rat Whole Genome Bioarray |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA124729 |
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