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| Status |
Public on Jan 01, 2023 |
| Title |
Gene expression analyses of HUVECs treated with conditioned medium derived from bone marrow mesenchymal stromal cells (BMSC-CM) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A functional vascular system is a prerequisite for bone repair as disturbed angiogenesis often causes non-union. Paracrine factors released from human bone marrow mesenchymal stromal cells (BMSCs) have angiogenic effects on endothelial cells. However, whether these paracrine factors participate in blood flow dynamics within bone capillaries remains poorly understood. Here, we used two different microfluidic designs to investigate critical steps during angiogenesis and found pronounced effects of endothelial cell proliferation as well as chemotactic and mechanotactic migration induced by BMSC conditioned medium (CM). The application of BMSC-CM in dynamic cultures demonstrates that bioactive factors in combination with fluidic flow-induced biomechanical signals significantly enhanced endothelial cell migration. Transcriptional analyses of endothelial cells demonstrate the induction of a unique gene expression profile related to tricarboxylic acid cycle and energy metabolism by the combination of BMSC-CM factors and shear stress, which opens an interesting new avenue to explore during fracture healing. Our results stress the importance of in vivo - like microenvironments simultaneously including biochemical, biomechanical and oxygen levels when investigating key events during vessel repair.
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| Overall design |
HUVECs were incubated with BMSC-CM in static culture and dynamic culture for 24 hours and gene expression profiles were generated.
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| Contributor(s) |
Zhang S, van de Peppel J, van der Eerden B |
| Citation(s) |
35995408 |
| |
| Submission date |
Jul 16, 2022 |
| Last update date |
Apr 02, 2023 |
| Contact name |
Jeroen van de Peppel |
| E-mail(s) |
h.vandepeppel@erasmusmc.nl
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| Organization name |
ErasmusMC
|
| Street address |
's Gravendijkwal 230
|
| City |
Rotterdam |
| ZIP/Postal code |
3015 CE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA859380 |
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