|
| Status |
Public on Nov 16, 2023 |
| Title |
Functional analysis of human cardiac enhancers by tiling mutagenesis |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
To further determine the sequence features of active cardiac enhancers, 123 cardiac enhancers were functionally dissected by 10 bp tiling deletion-based mutagenesis MPRA in iPSC-CMs.
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| Overall design |
We performed systematic, tiling mutagenesis MPRA of 123 cardiac enhancers identified by 400 bp lentiMPRA. Each 400 bp region was represented as three fragments (F1, F2, F3), each containing 171 bp of genomic sequence. Each of these wild type sequences was tiled with 17 10 bp deletions.
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| Contributor(s) |
Xiao F, Zhang X, Morton SU, Seidman CE, Seidman JG, Pu WT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 15, 2022 |
| Last update date |
Nov 17, 2023 |
| Contact name |
Xiaoran Zhang |
| E-mail(s) |
xiaoran.zhang@childrens.harvard.edu
|
| Phone |
8574259855
|
| Organization name |
Boston Children's Hospital
|
| Department |
Cardiology
|
| Lab |
Pu lab
|
| Street address |
14 Perkins Square, Apt 11
|
| City |
Jamaica Plain |
| State/province |
MA |
| ZIP/Postal code |
02130 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| GSM6340178 |
Mutagenesis MPRA in iPSC-CMs day 24 DNA-Rep-1 |
| GSM6340179 |
Mutagenesis MPRA in iPSC-CMs day 24 DNA-Rep-2 |
| GSM6340180 |
Mutagenesis MPRA in iPSC-CMs day 24 DNA-Rep-3 |
| GSM6340181 |
Mutagenesis MPRA in iPSC-CMs day 24 DNA-Rep-4 |
| GSM6340182 |
Mutagenesis MPRA in iPSC-CMs day 24 RNA-Rep-1 |
| GSM6340183 |
Mutagenesis MPRA in iPSC-CMs day 24 RNA-Rep-2 |
| GSM6340184 |
Mutagenesis MPRA in iPSC-CMs day 24 RNA-Rep-3 |
| GSM6340185 |
Mutagenesis MPRA in iPSC-CMs day 24 RNA-Rep-4 |
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| This SubSeries is part of SuperSeries: |
| GSE208286 |
Analysis of human iPSC-derived cardiomyocytes |
|
| Relations |
| BioProject |
PRJNA859125 |
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