|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Dec 02, 2022 |
Title |
Analysis of Polycomb Repressive Complex 1 subunits across cell cycle in pluripotent cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Other
|
Summary |
We report ChIP-seq of PRC1 proteins and 4sU-seq, mRNA-seq, Capture-C and 4C-seq data using mouse embryonic stem cells sorted in different phases of the cell cycle.
|
|
|
Overall design |
ChIP-seq samples for Ring1b, Rybp, H2AK119ub1 and Cbx7 obtained from cell-cycle sorted FUCCI-mESCs (G1, S, G2 fractions). 4sU-seq data in cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs. mRNA-seq data from cell cycle-sorted AID::Ring1b;FUCCI mESCs plated in differentiation media with IAA or without IAA during 6h. Capture-C data from G1 and G2 cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs. 4C-seq data from G1 and G2 cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs.
|
|
|
Contributor(s) |
Asenjo HG, Espinosa M, López-Onieva L, Alcazar-Fabra M, Gallardo A, Dimitrova E, Feldmann A, Pachano T, Martorell-Marugan J, Carmona-Saez P, Sanchez-Pozo A, Rada-Iglesias Á, Klose RJ, Landeira D |
Citation(s) |
36635295 |
|
Submission date |
Jul 12, 2022 |
Last update date |
Jun 30, 2023 |
Contact name |
Jordi Martorell-Marugan |
Organization name |
GENyO
|
Department |
Bioinformatics Unit
|
Street address |
Avenida de la Ilustración 114
|
City |
Granada |
ZIP/Postal code |
18016 |
Country |
Spain |
|
|
Platforms (3) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
GPL30172 |
NextSeq 2000 (Mus musculus) |
|
Samples (53)
|
|
Relations |
BioProject |
PRJNA857985 |
Supplementary file |
Size |
Download |
File type/resource |
GSE207997_RAW.tar |
36.5 Gb |
(http)(custom) |
TAR (of BEDGRAPH, BIGWIG, TAR) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
|
|
|
|
|