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Series GSE207997 Query DataSets for GSE207997
Status Public on Dec 02, 2022
Title Analysis of Polycomb Repressive Complex 1 subunits across cell cycle in pluripotent cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We report ChIP-seq of PRC1 proteins and 4sU-seq, mRNA-seq, Capture-C and 4C-seq data using mouse embryonic stem cells sorted in different phases of the cell cycle.
Overall design ChIP-seq samples for Ring1b, Rybp, H2AK119ub1 and Cbx7 obtained from cell-cycle sorted FUCCI-mESCs (G1, S, G2 fractions). 4sU-seq data in cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs. mRNA-seq data from cell cycle-sorted AID::Ring1b;FUCCI mESCs plated in differentiation media with IAA or without IAA during 6h. Capture-C data from G1 and G2 cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs. 4C-seq data from G1 and G2 cell cycle-sorted FUCCI-Ring1bfl/fl and FUCCI-Ring1b-/- mESCs.
Contributor(s) Asenjo HG, Espinosa M, López-Onieva L, Alcazar-Fabra M, Gallardo A, Dimitrova E, Feldmann A, Pachano T, Martorell-Marugan J, Carmona-Saez P, Sanchez-Pozo A, Rada-Iglesias Á, Klose RJ, Landeira D
Citation(s) 36635295
Submission date Jul 12, 2022
Last update date Jun 30, 2023
Contact name Jordi Martorell-Marugan
Organization name GENyO
Department Bioinformatics Unit
Street address Avenida de la Ilustración 114
City Granada
ZIP/Postal code 18016
Country Spain
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (53)
GSM6329684 Ring1b-G1
GSM6329685 Ring1b-S
GSM6329686 Ring1b-G2
BioProject PRJNA857985

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Supplementary file Size Download File type/resource
GSE207997_RAW.tar 36.5 Gb (http)(custom) TAR (of BEDGRAPH, BIGWIG, TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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