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| Status |
Public on Dec 15, 2022 |
| Title |
Early activated ageing-related changes in cardiac transcriptome of Tgαq*44 mice during entire HF development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate ageing-related changes in cardiac transcriptome of FVB mice we performed differential gene expression profiling analysis using data obtained from RNA-seq of five life time points (4-, 8-, 10-, 12-, 14 months) by comparing older FVB mice (8-, 10-, 12-, 14 months) to young 4-month-old FVB mice (FVB vs. FVB analysis). Genes that were differentially expressed in FVB vs. FVB analysis were also annotated to the respective biological processes based on Gene Ontology database, assessed for their overrepresentation to indicate processes involved in cardic ageing process in FVB mice. To investigate heart failure-related as well as ageing-related changes in cardiac transcriptome of Tgαq*44 mice we performed differential gene expression profiling analysis using data obtained from RNA-seq of five life time points (4-, 8-, 10-, 12-, 14 months) by comparing Tgαq*44 to age-matched FVB mice (Tgαq*44 vs. FVB analysis). Genes that were differentially expressed in Tgαq*44 vs. FVB analysis were also annotated to the respective biological processes based on Gene Ontology database, assessed for their overrepresentation to indicate processes iniciated along heart failure development as well as cardiac ageing process in Tgαq*44 mice. To investigate early activated ageing-related changes in cardiac transcriptome of Tgαq*44 mice during entire heart failure development we searched for the presence of ageing-related genes (those identified in cardiac transcriptome of older FVB mice) among genes which were differentially expressed commonly at each measured time points in Tgαq*44 vs. FVB analysis. To investigate early activated aging-related biological processes in cardiac transcriptome of Tgαq*44 mice during entire heart failure development, we searched for the presence of ageing-related processes (those identified in cardiac transcriptome of older FVB mice) among processes which were overrepresented commonly at each measured time points in Tgαq*44 vs. FVB analysis.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for older FVB mice (8-, 10-, 12-, 14 months) to young 4-month-old FVB mice as well as Tgαq*44 to age-matched FVB mice at the age of 4-, 8-, 10-, 12-, 14 months.
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| Contributor(s) |
Berkowicz P, Chlopicki S |
| Citation(s) |
36692592 |
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| Submission date |
Jul 07, 2022 |
| Last update date |
Mar 17, 2023 |
| Contact name |
Piotr Berkowicz |
| E-mail(s) |
piotr.berkowicz@jcet.eu
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| Organization name |
Jagiellonian Centre for Experimental Therapeutics
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| Street address |
Bobrzyńskiego 14
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| City |
Cracow |
| ZIP/Postal code |
30-348 |
| Country |
Poland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (50)
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| GSM6304317 |
FVB, 4 months, mouse 4 |
| GSM6304318 |
FVB, 4 months, mouse 5 |
| GSM6304319 |
FVB, 8 months, mouse 1 |
| GSM6304320 |
FVB, 8 months, mouse 2 |
| GSM6304321 |
FVB, 8 months, mouse 3 |
| GSM6304322 |
FVB, 8 months, mouse 4 |
| GSM6304323 |
FVB, 8 months, mouse 5 |
| GSM6304324 |
FVB, 10 months, mouse 1 |
| GSM6304325 |
FVB, 10 months, mouse 2 |
| GSM6304326 |
FVB, 10 months, mouse 3 |
| GSM6304327 |
FVB, 10 months, mouse 4 |
| GSM6304328 |
FVB, 10 months, mouse 5 |
| GSM6304329 |
FVB, 12 months, mouse 1 |
| GSM6304330 |
FVB, 12 months, mouse 2 |
| GSM6304331 |
FVB, 12 months, mouse 3 |
| GSM6304332 |
FVB, 12 months, mouse 4 |
| GSM6304333 |
FVB, 12 months, mouse 5 |
| GSM6304334 |
FVB, 14 months, mouse 1 |
| GSM6304335 |
FVB, 14 months, mouse 2 |
| GSM6304336 |
FVB, 14 months, mouse 3 |
| GSM6304337 |
FVB, 14 months, mouse 4 |
| GSM6304338 |
FVB, 14 months, mouse 5 |
| GSM6304339 |
Tgαq*44, 4 months, mouse 1 |
| GSM6304340 |
Tgαq*44, 4 months, mouse 2 |
| GSM6304341 |
Tgαq*44, 4 months, mouse 3 |
| GSM6304342 |
Tgαq*44, 4 months, mouse 4 |
| GSM6304343 |
Tgαq*44, 4 months, mouse 5 |
| GSM6304344 |
Tgαq*44, 8 months, mouse 1 |
| GSM6304345 |
Tgαq*44, 8 months, mouse 2 |
| GSM6304346 |
Tgαq*44, 8 months, mouse 3 |
| GSM6304347 |
Tgαq*44, 8 months, mouse 4 |
| GSM6304348 |
Tgαq*44, 8 months, mouse 5 |
| GSM6304349 |
Tgαq*44, 10 months, mouse 1 |
| GSM6304350 |
Tgαq*44, 10 months, mouse 2 |
| GSM6304351 |
Tgαq*44, 10 months, mouse 3 |
| GSM6304352 |
Tgαq*44, 10 months, mouse 4 |
| GSM6304353 |
Tgαq*44, 10 months, mouse 5 |
| GSM6304354 |
Tgαq*44, 12 months, mouse 1 |
| GSM6304355 |
Tgαq*44, 12 months, mouse 2 |
| GSM6304356 |
Tgαq*44, 12 months, mouse 3 |
| GSM6304357 |
Tgαq*44, 12 months, mouse 4 |
| GSM6304358 |
Tgαq*44, 12 months, mouse 5 |
| GSM6304359 |
Tgαq*44, 14 months, mouse 1 |
| GSM6304360 |
Tgαq*44, 14 months, mouse 2 |
| GSM6304361 |
Tgαq*44, 14 months, mouse 3 |
| GSM6304362 |
Tgαq*44, 14 months, mouse 4 |
| GSM6304363 |
Tgαq*44, 14 months, mouse 5 |
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| Relations |
| BioProject |
PRJNA856618 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207648_156_common_DEGs_for_4-14months_Tgvs.FVB.xlsx |
45.4 Kb |
(ftp)(http) |
XLSX |
| GSE207648_DEGs_FVB_vs_FVB_analysis.xlsx |
229.5 Kb |
(ftp)(http) |
XLSX |
| GSE207648_DEGs_Tg_vs_FVB_analysis.xlsx |
751.1 Kb |
(ftp)(http) |
XLSX |
| GSE207648_ageing_genes_in_HF_Tg_vs_FVB_analysis_.xlsx |
264.5 Kb |
(ftp)(http) |
XLSX |
| GSE207648_ageing_processes_in_HF_Tg_vs_FVB_analysis_.xlsx |
84.7 Kb |
(ftp)(http) |
XLSX |
| GSE207648_annotated_DEGs_to_overrepresented_processes_in_FVBvsFVB.xlsx |
100.8 Kb |
(ftp)(http) |
XLSX |
| GSE207648_annotated_DEGs_to_overrepresented_processes_in_Tgvs.FVB.xlsx |
298.8 Kb |
(ftp)(http) |
XLSX |
| GSE207648_counts_matrix.xlsx |
11.1 Mb |
(ftp)(http) |
XLSX |
| GSE207648_gene_freq_in_common_overrepresented_processes_4-14_months_TgvsFVB.xlsx |
72.4 Kb |
(ftp)(http) |
XLSX |
| GSE207648_genes_and_processes_ageing_heart_FVBvs.FVB.xlsx |
62.9 Kb |
(ftp)(http) |
XLSX |
| GSE207648_genes_and_processes_of_aged_heart_FVBvs.FVB.xlsx |
64.1 Kb |
(ftp)(http) |
XLSX |
| GSE207648_overrepresented_processes_FVB_vs_FVB_analysis.xlsx |
73.0 Kb |
(ftp)(http) |
XLSX |
| GSE207648_overrepresented_processes_Tg_vs_FVB_analysis.xlsx |
270.8 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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