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| Status |
Public on Apr 01, 2024 |
| Title |
Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing [scRNA-seq cocultured HSPCs] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single cell RNA-seq analysis was performed on sorted population of LSK HSPCs with different genetic background and phenotype, after co-culture with vascular niche cells, to uncover differential transcriptional output following genetic manipulation.
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| Overall design |
LSK HSPCs were harvested isolated and sorted from flushed and crushed murine tibias and femurs. LSK HSPCs (WT, Fli-1ROSAΔ , Fli-1ROSAΔN1-ICiOE, and N1-ICiOE) were introduced into co-culture with vascular niche ECs. 4-OHT was introduced into cultures to induce genetic manipulation. Cultures were harvested and LSK HSPCs were resorted and processed for 10X Chromium scRNA-seq analysis.
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| Contributor(s) |
Itkin T, Houghton S, Rafii S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 06, 2022 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Tomer Itkin |
| E-mail(s) |
toi2003@med.cornell.edu
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| Organization name |
Weill Cornell Medicine
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| Department |
Medicine
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| Lab |
Rafii
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| Street address |
1300 York Avenue, A-869
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| City |
NY |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE169431 |
Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing |
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| Relations |
| BioProject |
PRJNA856257 |
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