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| Status |
Public on Jul 01, 2024 |
| Title |
The immune landscape and mechanism of hepatitis B virus surface antigen clearance [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hepatitis B virus (HBV) surface antigen (HBsAg) clearance constitutes the hallmark of resolution of acute infection and is a therapeutic goal for functional cure of chronic hepatitis B. Here, we reveal the immunological landscape and mechanism of HBsAg clearance in mice. After acquiring nonopsonized HBsAg, B cells prime CD4+ T cell responses by CCR5- and EBI2-guided interaction with CD4+ T cells at follicular and interfollicular regions, respectively. Monocyte-derived macrophages transport complement-opsonized HBsAg to follicular dendritic cells (FDCs) to maintain germinal center reaction. Batf3+ XCR1+ conventional type 1 DCs (cDC1s) acquire and present HBsAg by MHC-I cross-dressing to drive CD8+ T cell responses in liver. We map the antigen-presenting cell (APC)-T cell crosstalk landscape and identify key costimulatory signals. The immune responses in patients with acute HBV infection are revealed by a single-cell transcriptome atlas. These findings revolutionize our understanding of anti-HBV immune responses and would lead to immunotherapeutic strategies for HBsAg clearance.
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| Overall design |
HBsAg-specific S129G1HL B cells with/without co-transferred OT-II T cells in the spleens were FACS-sorted from mice at 0 h, 18 h, day 1 and day 2.5 after pHBV1.3/OVA323-339 hydrodynamically injection, and used for RNAseq.
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| Contributor(s) |
Li X, Sun W, Xu X, Bai L, Jiang Q, Jin C, Liu J, Shi B, Zhang J, Yuan Z, Li J |
| Citation missing |
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| |
| Submission date |
Jul 06, 2022 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Jianhua Li |
| E-mail(s) |
jianhuali@fudan.edu.cn
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| Organization name |
Fudan University
|
| Street address |
NO.131 DONGAN RD., FUXING BUILDING 306
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE207543 |
The immune landscape and mechanism of hepatitis B virus surface antigen clearance |
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| Relations |
| BioProject |
PRJNA856138 |
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