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| Status |
Public on Jul 04, 2022 |
| Title |
Pregnancy-induced maternal microchimerism shapes neurodevelopment and behavior in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Maternal immune cells (called 'Maternal microchimeric cells' (short: MMc)) seed fetal brain tissue during pregnancy. We used single cell RNA sequencing (scRNA-seq) to characterize the diversity of MMc in the fetal brain.
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| Overall design |
C57BL/6 female mice were mated to CByJ.SJL(B6)-Ptprca/J male mice. MMc were isolated from 16 fetal brains from 4 litters by Fluorescence-activated cell sorting (FACS) according to CD45.2 and H-2Db/b expression and analyzed using scRNAseq.
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| Contributor(s) |
Schepanski S, Sun T, Zhao Y, Woestemeier A, Bonn S, Gagliani N, Hanganu-Opatz IL, Arck PC |
| Citation(s) |
35931682 |
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| Submission date |
Jul 01, 2022 |
| Last update date |
Aug 24, 2022 |
| Contact name |
Yu Zhao |
| E-mail(s) |
yu.zhao@zmnh.uni-hamburg.de
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| Organization name |
University Medical Center Hamburg-Eppendorf
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| Department |
Institute of Medical Systems Biology
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| Street address |
Falkenried 94
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| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA854825 |
