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| Status |
Public on Oct 04, 2022 |
| Title |
SHARE-seqV2 for single-cell epigenomic and transcriptomic mapping of human hematopoiesis at scale |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
A growing body of single-cell multi-omic tools enables a better understanding of gene regulation underlying development and disease. Tools mapping chromatin and gene expression (such as SHARE-seq) are limited in sensitivity and scalability. Here, we describe SHARE-seqV2 and its associated cloud-computing resource, enabling single-cell analysis at improved quality, robustness, and scale. We apply SHARE-seqV2 across various cell lines, primary tissues, and immune cells, demonstrating a generalizable platform for single-cell multi-omics.
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| Overall design |
SHARE-seqV2 to profile chromatin accessibility and gene expression in the same cell from cell lines (HEK and 3T3), mouse colon, cultured HSC, and primey BMMC.
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| Contributor(s) |
Ma S, Buenrostro J |
| Citation missing |
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| |
| Submission date |
Jun 30, 2022 |
| Last update date |
Dec 09, 2022 |
| Contact name |
Sai Ma |
| E-mail(s) |
masai.zju@gmail.com
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| Organization name |
Icahn School of Medicine at Mount Sinai
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| Street address |
1425 Madison Ave
|
| City |
New York |
| ZIP/Postal code |
10026 |
| Country |
USA |
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| Platforms (3) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA854664 |
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