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| Status |
Public on Apr 03, 2024 |
| Title |
Single-Nucleus Transcriptomic Atlas of Goat (Capra hircus) Ovarian Prolificacy II |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Molecular mechanisms of follicular atresia and prolificacy of mammal remain unclear. We surveyed the single-cell transcriptomic landscape of ovaries from single and prolificacy goat and identified seven ovarian cell types with distinct gene-expression, transcriptional factor networks and reciprocal interactions signatures. In-depth dissection of gene-expression dynamics of granulosa cells (GCs) that displayed development stage-specific expression patterns and specific gene signatures were identified that may reflect developmental competency and ovarian reserve. what’s more, we revealed the origin of theca cells. Further analysis of cell-type-specific prolificacy-associated transcriptional changes uncovered apoptosis, anabolism and response to hormone stimulation as are crucial factor in dominant follicle development and ovulation. Additionally, differentially expressed genes (DEGs) of SERPINE2 can interact with CYP19A1 to promote cell proliferation, inhibit apoptosis and promoting the anabolism were observed in mouse granulosa cells. Thus, our work provides a comprehensive understanding of the cell-type-specific mechanisms underlying goat ovarian prolificacy at single-cell resolution, provides key insights into offers important clues for improving follicle recruitment in vivo and revealing new diagnostic biomarkers and potential therapeutic targets for ovulation disorder.
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| Overall design |
Ovarian tissue samples from 2 Nubian goats (about 3 years old) and 2 Du’an goat (about 3 years old) were collected and processed for snRNA-seq analysis.
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| Contributor(s) |
Jiang Q, Huang Y, Zhang S |
| Citation(s) |
38534763 |
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| Submission date |
Jun 27, 2022 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Sanbao Zhang |
| E-mail(s) |
gxuzsb@126.com
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| Phone |
18447055384
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| Organization name |
Guangxi University
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| Street address |
No.100,Daxue East Road
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| City |
Nanning |
| ZIP/Postal code |
530004 |
| Country |
China |
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| Platforms (1) |
| GPL27106 |
Illumina NovaSeq 6000 (Capra hircus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA853333 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207023_RAW.tar |
234.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE207023_all_marker_for_GCs_during_differentiation.xlsx |
911.8 Kb |
(ftp)(http) |
XLSX |
| GSE207023_all_marker_for_each_follicle_GCs_during_follicle_development.xlsx |
1.1 Mb |
(ftp)(http) |
XLSX |
| GSE207023_all_markers_for_each_cell_type.xlsx |
2.0 Mb |
(ftp)(http) |
XLSX |
| GSE207023_all_markers_for_each_cluster_anno_GCs_during_differentiation.xls.gz |
685.4 Kb |
(ftp)(http) |
XLS |
| GSE207023_all_markers_for_each_cluster_anno_GCs_during_follicle_development.xls.gz |
919.6 Kb |
(ftp)(http) |
XLS |
| GSE207023_gene_of_each_module_for_external_theca_cells.xlsx |
70.9 Kb |
(ftp)(http) |
XLSX |
| GSE207023_genes_of_each_module_for_GCs_during_follicle_development.xlsx |
171.6 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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