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Series GSE206790 Query DataSets for GSE206790
Status Public on Apr 10, 2024
Title Profibrotic subsets of SPP1+ macrophages and POSTN+ fibroblasts contribute to fibrotic scarring in acne keloidalis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Acne keloidalis (AK) is a primary scarring alopecia characterized by longstanding inflammation in the scalp leading to keloid-like scar formation and hair loss. Histologically, AK is characterized by mixed leukocytic infiltrates in the acute stage followed by a granulomatous reaction and extensive fibrosis in later stages. To further explore its pathogenesis, bulk RNA-sequencing and single-cell RNA sequencing were applied to scalp biopsy specimens of lesional and adjacent non-lesional skin in patients with clinically active disease. Unbiased clustering revealed 18 distinct cell populations, including two notable populations, POSTN+ fibroblasts with enriched extracellular matrix signatures, and SPP1+ myeloid cells M2 macrophages. Cell communication analyses indicate that fibroblasts and myeloid cells communicate by collagen and SPP1 signaling networks in lesional skin. Tissue immunofluorescence staining demonstrated SPP1+ myeloid cells and POSTN+ fibroblasts at the upper segment of outer root sheath of the hair follicle in the subacute stage, confirming micro-anatomic specificity with relevant disease activity. Therapy with intralesional corticosteroids reduced SPP1 and POSTN expression, and lessened AK progression. In summary, the communication between POSTN+ fibroblasts and SPP1+ myeloid cells by collagen and SPP1 axis may contribute to the pathogenesis of AK.
Overall design We conducted droplet-based scRNA-seq (10X Genomics, California, USA) on paired 6 mm punch biopsies of AK lesional skin (n = 1) and adjacent healthy non-lesional skin (n = 1) from an individual patient
Please note that raw data are not available due to patient privacy concerns.
Contributor(s) Hong Y, Hsu C
Citation(s) 38218364
Submission date Jun 23, 2022
Last update date Apr 11, 2024
Contact name Yi-Kai Hong
Phone 0623535355542
Organization name National Cheng Kung University
Street address No.1, Daxue Rd., East Dist.
City Tainan City
State/province Please select an option below
ZIP/Postal code 70101
Country Taiwan
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM7880108 Skin, Nonlesional, rep1
GSM7880109 Skin, Lesional, rep1
GSM7880110 Skin, Lesional, rep2
BioProject PRJNA852200

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Supplementary file Size Download File type/resource
GSE206790_RAW.tar 157.3 Mb (http)(custom) TAR (of TAR)
GSE206790_processed_data_file_RNA-seq.csv.gz 795.9 Kb (ftp)(http) CSV
Raw data not provided for this record
Processed data provided as supplementary file

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