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| Status |
Public on Feb 01, 2023 |
| Title |
Mapping the Spatial Dynamics of the Human Oral Mucosa in Chronic Inflammatory Disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The interplay among different cells in a tissue is essential for maintaining homeostasis. Although, disease states have been traditionally attributed to individual cell types, increasing evidence and new therapeutic options have demonstrated the primary role of multicellular functions to understand health and disease, opening new avenues to understand pathogenesis and develop new treatment strategies. We recently described the cellular composition and dynamics of the human oral mucosa; however, the spatial arrangement of cells is needed to better understand a morphologically complex tissue. Here, we link single-cell RNA sequencing, spatial transcriptomics, and high-resolution multiplex fluorescence in situ hybridisation to characterise human oral mucosa in health and oral chronic inflammatory disease. We define highly specialised epithelial and stromal compartments and describe location-specific immune programs. Furthermore, we discovered fine regional fibroblast states, and spatially mapped a rare pathogenic population localised in a highly immunogenic region, responsible for lymphocyte recruitment through CXCL8 and CXCL10 and with a possible role in pathological angiogenesis through ALOX5AP. Collectively, our study provides a comprehensive reference for the study of oral chronic disease pathogenesis.
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| Overall design |
Adult oral mucosa tissue sections from healthy and periodontitis affected individuals were subjected to spatial transcriptomics analyses.
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| Contributor(s) |
Caetano AJ, Redhead Y, Karim F, Dhami P, Kannambath S, Nuamah R, Volponi AA, Nibali L, Booth V, D'Agostino EM, Sharpe PT |
| Citation(s) |
36648332 |
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| Submission date |
Jun 22, 2022 |
| Last update date |
Feb 02, 2023 |
| Contact name |
Paul Sharpe |
| E-mail(s) |
paul.sharpe@kcl.ac.uk
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| Organization name |
King's College London
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| Department |
Centre for Craniofacial and Regenerative Biology
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| Lab |
Sharpe
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| Street address |
Guy's Hospital
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| GSM6258251 |
Spatial transcriptomics cryo technical replicate 1 [A1] |
| GSM6258252 |
Spatial transcriptomics cryo technical replicate 2 [B1] |
| GSM6258253 |
Spatial transcriptomics cryo technical replicate 1 [C1] |
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| Relations |
| BioProject |
PRJNA851592 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206621_RAW.tar |
127.5 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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