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| Status |
Public on Apr 01, 2010 |
| Title |
Identification and validation of the pathways and functions regulated by the ROR alpha1 in skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Identification and validation of the pathways and functions regulated by the orphan nuclear receptor, ROR alpha1, in skeletal muscle
The retinoic acid receptor-related orphan receptor (ROR) alpha has been demonstrated to regulate lipid metabolism. We were interested in the physiologically relevant roles, and pathways regulated by RORalpha1 action in skeletal muscle. This major mass organ accounts for ~40% of the total body mass, and significant levels of lipid catabolism, glucose disposal and energy expenditure. We utilized the strategy of targeted muscle specific expression of a truncated (dominant negative) RORalpha∆DE in transgenic mice, to investigate RORalpha1 signalling (and function) in this peripheral tissue. Expression profiling and pathway analysis indicated that RORalpha regulated genes involved in: (i) lipid and carbohydrate metabolism, cardiovascular and metabolic disease; and (ii) the LXR nuclear receptor signaling pathway and, (iii) the Akt and AMPK signaling cascades. This analysis was extensively validated by rigorous qPCR analysis using TaqMan Low Density Arrays, coupled to rigorous statistical analysis (with Empirical Bayes, and Benjamini-Hochberg). Moreover, westerns and metabolic profiling were utilized to validate the genes, proteins and pathways (lipogenic, Akt, AMPK and fatty acid oxidation) involved in the regulation of metabolism by RORalph1. The identified genes and pathways were in concordance with the demonstration of hyperglycemia, glucose intolerance, attenuated insulin stimulated phosphorylation of Akt, and impaired glucose uptake in the transgenic heterozygous Tg-RORalpha∆DE animals. In conclusion, we propose that RORalpha1 is involved in regulating the Akt2-AMPK signalling pathways in the context of lipid homeostasis in skeletal muscle.
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| Overall design |
Total RNA was compared from quadriceps femoris of both transgenic and wild type mice. Transgenic mice contained a truncated version of human RORalpha1 (RORalpha1delDE) where the entire E region and part of the hinge/D region have been removed. This transgene is driven by a skeletal muscle specific human skeletal alpha-actin (HSA) promoter.
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| Contributor(s) |
Raichur S, Fitzsimmons RL, Myers SA, Pearen MA, Lau P, Eriksson NA, Wang SM, Muscat GE |
| Citation(s) |
20338882 |
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| Submission date |
Mar 05, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Michael Andrew Pearen |
| E-mail(s) |
m.pearen@uq.edu.au
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| Phone |
+61 7 33462365
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| Fax |
+61 7 33462101
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| Organization name |
Institute for Molecular Bioscience
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| Lab |
Group Muscat
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| Street address |
The University of Queensland
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| City |
St Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA124869 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20646_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR |
| GSE20646_non-normalized.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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