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| Status |
Public on Jul 14, 2022 |
| Title |
Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We conducted an animal experiment and used total RNA sequencing (RNA-Seq) to identify novel RNA markers to detect autologous blood transfusion (ABT) doping within red blood cells (RBCs) as a pilot study before human trials.
The total RNA-Seq and bioinformatics were performed using rat RBCs that were stored 10- and 20-day periods with citrate–phosphate–dextrose solution with adenine (CPDA) at 4°C
In the results, we identified three significant genes whose expression levels were drastically decreased according to the storage period.
Our novel insights may allow future studies to develop a test method for ABT doping.
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| Overall design |
RNAs of RBCs purified form the whole bloods were analyzed using total RNA-Seq, at each storage period.
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| Contributor(s) |
Sugasawa T |
| Citation(s) |
35886040 |
| |
| Submission date |
Jun 10, 2022 |
| Last update date |
Aug 03, 2022 |
| Contact name |
takehito sugasawa |
| E-mail(s) |
takekoshi2020@gmail.com
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| Phone |
+81-29-853-3209
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| Organization name |
University of tsukuba
|
| Street address |
Ibaraki
|
| City |
Tsukuba |
| State/province |
Select State |
| ZIP/Postal code |
3058577 |
| Country |
Japan |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA847776 |
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