|
| Status |
Public on Oct 06, 2022 |
| Title |
p300/CBP sustains Polycomb silencing by non-enzymatic functions (RNA-seq on inhibitor-treated Drosophila S2 cells) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Maintenance of appropriate cell states involves epigenetic mechanisms, including Polycomb group (PcG)-mediated transcriptional repression. While PcG proteins are known to induce chromatin compaction, how PcG proteins gain access to DNA in compact chromatin to achieve long-term silencing is poorly understood. Here we show that the p300/CBP co-activator is associated with two-thirds of PcG-regions and required for PcG occupancy at many of these in Drosophila and mouse cells. CBP stabilizes RNA polymerase II (Pol II) at PcG-bound repressive sites and promotes Pol II pausing independently of its histone acetyltransferase activity. CBP and Pol II pausing are necessary for RNA-DNA hybrid (R-loop) formation and nucleosome depletion at Polycomb Response Elements (PREs), whereas transcription beyond the pause region is not. These results suggest that non-enzymatic activities of the CBP co-activator have been repurposed to support PcG-mediated silencing, revealing how chromatin regulator interplay maintains transcriptional states.
|
| |
|
| Overall design |
RNA-seq analysis of gene expression changes in Drosophila late embryonic Schneider 2 cells treated for 30 minutes with the CBP HAT pharmacological inhibitor C646 with and without a drug washout (WO) of 1 hr or 4 hr, relative to untreated cells.
|
| |
|
| Contributor(s) |
Hunt G, Boija A, Mannervik M |
| Citation(s) |
36206738 |
| |
| Submission date |
Jun 07, 2022 |
| Last update date |
Oct 12, 2022 |
| Contact name |
Mattias Mannervik |
| E-mail(s) |
mattias.mannervik@su.se
|
| Organization name |
Stockholm University
|
| Department |
Dept. of Molecular Biosciences
|
| Street address |
Svante Arrhenius väg 20C
|
| City |
Stockholm |
| ZIP/Postal code |
106 91 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL30203 |
NextSeq 2000 (Drosophila melanogaster) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE186723 |
p300/CBP sustains Polycomb silencing by non-enzymatic functions |
|
| Relations |
| BioProject |
PRJNA846733 |
Less...
More...