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Series GSE205542 Query DataSets for GSE205542
Status Public on Jun 13, 2022
Title Selective PROTAC-mediated degradation and shRNA-driven knockdown of SMARCA2 in HCC2302 lung adenocarcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary SMARCA2 and SMARCA4 are mutually-exclusive, core catalytic subunits for the chromatin remodeling SWI/SNF complex. Mutations in SMARCA4 are common in lung adenocarcinoma and have shown synthetic lethality with loss of SMARCA2 loss of function. The purpose of this project is to answer questions related to the in vivo [mouse xenograft] efficacy of A947, a SMARCA2 degrader. The study involves in vitro versus in vivo comparisons of drug activity, in addition to genetic manipulation of the target. Model system: HCC2302 (RRID:CVCL_V636); Model origin: Lung Adenocarcinoma; Perturbation: A947 (VHL-PROTAC-based degrader)
 
Overall design in vitro samples are treated with either a non-targeting shRNA (in vitro HCC2302-shNTC) or a SMARCA2-targeting shRNA (in vitro HCC2302-shSMARCA2-dual62 cl2), both of which are doxycycline-inducible. Vehicle (non-induced) samples are labeled as "Veh" and doxycycline-induced samples are labeled as "Dox." in vivo mouse xenograft samples (in vivo mouse xenograft HCC2302) are treated with either vehicle (Veh) or A947 (A947). There are 3 replicates per in vitro condition and 5 replicates per in vivo condition.
 
Contributor(s) Yauch B, Ye X, Grimmer MR
Citation(s) 36357397
Submission date Jun 06, 2022
Last update date Dec 01, 2022
Contact name Matthew R Grimmer
E-mail(s) grimmem1@gene.com
Organization name Genentech
Department Discovery Oncology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (22)
GSM6214463 in vitro HCC2302-shNTC Veh rep1
GSM6214464 in vitro HCC2302-shNTC Veh rep2
GSM6214465 in vitro HCC2302-shNTC Veh rep3
Relations
BioProject PRJNA846300

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Supplementary file Size Download File type/resource
GSE205542_counts_voomed.txt.gz 1.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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