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| Status |
Public on Feb 25, 2011 |
| Title |
Gene expression in response to axotomy in organotypical slices co-cultures |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Brain slice cultures offer advantages over other in vitro methods, as they mimic numerous in vivo aspects. For most purposes, slices of the developing brain, termed organotypic slice cultures , preserve a high degree of cellular differentiation and tissue organization. The entorhino-hippocampal connection (EHP) is the main entrance of information to the hippocampus proper and the dentate gyrus. Also it has some specific features that make them particularly interesting in studies of axonal regeneration: (i) the culture method obviates the need for extensive animal surgery and requires less time than other in vivo approaches; (ii) the EHP is reproduced easily in vitro in cultures with a degree of laminar specificity similar to that found in vivo; (iii) the EHP is myelinated both in vitro and in vivo; and (iv) most of the cellular and molecular barriers to axon regeneration are present after the axotomy of the EHP in vitro. Altogether this model is useful to evaluate axon regeneration and putative estrategies to promote axonal growth.
Keywords: organotypic slice cultures; axonal lession; gene expression
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| Overall design |
To evaluate the genes whose transcription was regulated after 1, 3 and 7 days after EHP (Entorhino-Hippocampal Pathway) axotomy, RNA samples were analyzed with Agilent whole genome rat long oligonucleotide (44 K base) probe based microarrays.
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| Contributor(s) |
Seira O, Llorens F, delRio JA, Rangel A, Sumoy L, Gavin R, Gil V |
| Citation(s) |
20374426 |
| |
| Submission date |
Feb 25, 2010 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Lauro Sumoy |
| E-mail(s) |
lsumoy@igtp.cat
|
| Organization name |
IGTP
|
| Department |
High Content Genomics and Bioinformatics
|
| Street address |
Ctra. Can Ruti, Camà de les escoles s/n
|
| City |
Badalona |
| State/province |
Barcelona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL2877 |
Agilent-013162 Whole Rat Genome Microarray G4131A (Feature Number version) |
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| Samples (12)
|
| GSM515650 |
1 day lesioned versus control array 1 |
| GSM515651 |
1 day lesioned versus control array 2 |
| GSM515652 |
1 day lesioned versus control array 3 |
| GSM515653 |
1 day lesioned versus control array 4 |
| GSM515654 |
3 day lesioned versus control array 1 |
| GSM515655 |
3 day lesioned versus control array 2 |
| GSM515656 |
3 day lesioned versus control array 3 |
| GSM515657 |
3 day lesioned versus control array 4 |
| GSM515658 |
7 day lesioned versus control array 1 |
| GSM515659 |
7 day lesioned versus control array 2 |
| GSM515660 |
7 day lesioned versus control array 3 |
| GSM515661 |
7 day lesioned versus control array 4 |
|
| Relations |
| BioProject |
PRJNA125143 |
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