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| Status |
Public on Dec 31, 2022 |
| Title |
ChIP-Seq of DNA lesions from irradiated and non-irradiated HaCaT cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study aims to investigate the binding DNA lesions of AIM2 in HaCaT cells exposed to 0Gy and 20Gy by ChIP-Seq. In this study, HaCaT cells were maintained in DMEM. All culture media were supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit-Haemek, Israel). Cells were grown at 37 °C in 5% CO2 incubators. Cells were exposed to 20Gy of ionizing radiation using X-ray linear accelerator (Rad Source, Suwanee, GA) at a fixed dose rate of 1.15 Gy/min.Four hours after irradiation, specific antibody against AIM2 was used to pull down AIM2 and the binding DNA lesions, which was then analyzed through ChIP-Seq.
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| Overall design |
The feature of AIM2 binding DNA lesions were compared through ChIP-Seq in HaCaT cells exposed to ionizing radiation or not.
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| Contributor(s) |
Zhang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 01, 2022 |
| Last update date |
Jan 02, 2023 |
| Contact name |
zhang shu yu |
| E-mail(s) |
zhang.shuyu@hotmail.com
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| Phone |
15851417273
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| Organization name |
Soochow University
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| Department |
soochow university
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| Lab |
radiation
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| Street address |
No. 199, Ren'ai Road
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| City |
suzhou |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA844273 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205250_RAW.tar |
114.6 Mb |
(http)(custom) |
TAR (of BW, XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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