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| Status |
Public on Jun 01, 2022 |
| Title |
miRNA expression in tracheal aspirates [sBPD, BPD-PH] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Extreme preterm infants are a growing population in the neonatal intensive care unit. Multiple factors play a role in preterm birth, resulting in complications including severe bronchopulmonary dysplasia (sBPD) without or with and pulmonary hypertension (BPD-PH). The goal of this study was to identify biomarker signatures associated with sBPD and BPD-PH. We analyzed profiles in tracheal aspirates (TAs) from 46 extremely preterm infants receiving invasive mechanical ventilation (25 sBPD, 21 BPD-PH) . We found specific miRNA signatures in TAs that may serve as biomarkers for the two disease phenotypes.
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| Overall design |
We extracted RNA from 0.5 mL of tracheal aspirates from premature neonates with BPD and BPD-PH using the Plasma/Serum RNA Purification Kit (Norgen). The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method.
variables: sBPD, BPD-PH
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| Web link |
https://onlinelibrary.wiley.com/doi/10.1002/pul2.12232
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| Contributor(s) |
Silveyra P, Siddaiah R, Oji-Mmuo C |
| Citation(s) |
37123538 |
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| Submission date |
May 31, 2022 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Patricia Silveyra |
| E-mail(s) |
psilveyr@iu.edu, ptrcslvr@gmail.com
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| Organization name |
Indiana University Bloomington
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| Department |
Environmental and Occupational Health
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| Lab |
Silveyra lab
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| Street address |
2819 E 10th St
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| City |
Bloomington |
| State/province |
Indiana |
| ZIP/Postal code |
47408 |
| Country |
USA |
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| Platforms (1) |
| GPL26552 |
Qiagen Human miRNome miScript miRNA PCR Array (MIHS-3216Z) |
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| Samples (46)
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| GSM6205642 |
BPD+PH, 4 |
| GSM6205643 |
BPD+PH, 6 |
| GSM6205644 |
BPD+PH, 7 |
| GSM6205645 |
sBPD, 11 |
| GSM6205646 |
sBPD, 12 |
| GSM6205647 |
sBPD, 13 |
| GSM6205648 |
BPD+PH, 16 |
| GSM6205649 |
BPD+PH, 23 |
| GSM6205650 |
sBPD, 24 |
| GSM6205651 |
sBPD, 26 |
| GSM6205652 |
BPD+PH, 28 |
| GSM6205653 |
BPD+PH, 31 |
| GSM6205654 |
sBPD, 33 |
| GSM6205655 |
BPD+PH, 34 |
| GSM6205656 |
sBPD, 37 |
| GSM6205657 |
sBPD, 42 |
| GSM6205658 |
BPD+PH, 45 |
| GSM6205659 |
BPD+PH, 46 |
| GSM6205660 |
sBPD, 48 |
| GSM6205661 |
sBPD, 49 |
| GSM6205662 |
sBPD, 51 |
| GSM6205663 |
sBPD, 52 |
| GSM6205664 |
BPD+PH, 55 |
| GSM6205665 |
sBPD, 56 |
| GSM6205666 |
BPD+PH, 58 |
| GSM6205667 |
sBPD, 59 |
| GSM6205668 |
sBPD, 60 |
| GSM6205669 |
BPD+PH, 62 |
| GSM6205670 |
BPD+PH, 63 |
| GSM6205671 |
BPD+PH, 64 |
| GSM6205672 |
BPD+PH, 66 |
| GSM6205673 |
sBPD, 67 |
| GSM6205674 |
BPD+PH, 70 |
| GSM6205675 |
sBPD, 74 |
| GSM6205676 |
BPD+PH, 76 |
| GSM6205677 |
sBPD, 77 |
| GSM6205678 |
sBPD, 80 |
| GSM6205679 |
BPD+PH, 84 |
| GSM6205680 |
sBPD, 91 |
| GSM6205681 |
BPD+PH, 93 |
| GSM6205682 |
sBPD, 95 |
| GSM6205683 |
sBPD, 96 |
| GSM6205684 |
sBPD, 100 |
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| Relations |
| BioProject |
PRJNA843988 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205138_RAW.tar |
30.0 Kb |
(http)(custom) |
TAR |
| GSE205138_fold-change_data.txt.gz |
206.6 Kb |
(ftp)(http) |
TXT |
| GSE205138_non-normalized_data.txt.gz |
198.4 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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