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| Status |
Public on Sep 29, 2022 |
| Title |
The Small RNA Teg41 Is a Pleiotropic Regulator of Virulence in Staphylococcus aureus [teg41 psm] |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Staphylococcus aureus is Gram-positive commensal bacteria that can also cause human disease ranging from mild, self-resolving skin infections to life-threatening conditions like endocarditis, osteomyelitis, and septicemia. Previously we demonstrated a role for the S. aureus sRNA, Teg41 in regulating production of the alpha Phenol Soluble Modulin toxins (αPSMs). In this study, we further characterize the regulatory role of Teg41. RNAseq analysis shows Teg41 influences the abundance of not just the αPSM transcript, but a variety of other transcripts as well compared to wild type S. aureus. Proteomic analysis confirms that eliminating Teg41 from the cell influences both the cytoplasmic and secreted protein profile of S. aureus. Finally, we observe that the Teg41Δ3′ strain is more severely attenuated in two murine infection models than a ∆αPSM strain, indicating that Teg41 regulation reaches beyond the αPSMs. Overall, we show that Teg41 is a unique pleiotropic sRNA in S. aureus that influences several key cellular processes.
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| Overall design |
6 datasets in total. Two strains (Teg41D3' strain and Dpsm mutant) with three replicates for each strain.
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| Contributor(s) |
Briaud P, Zapf RL, Mayher AD, Frey A, Shaw LN, Bose JL, Carroll RK |
| Citation(s) |
36214557 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI143743 |
Post-translational regulation of alpha PSM production in Staphylococcus aureus by the small RNA Teg41 |
OHIO UNIVERSITY |
Ronan Carroll |
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| |
| Submission date |
May 22, 2022 |
| Last update date |
Jan 08, 2023 |
| Contact name |
Ronan Kevin Carroll |
| E-mail(s) |
carrolr3@ohio.edu
|
| Phone |
740-593-2201
|
| Organization name |
Ohio University
|
| Department |
Biological Sciences
|
| Street address |
107 Irvine Hall
|
| City |
Athens |
| State/province |
OHIO |
| ZIP/Postal code |
45701 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL30817 |
NextSeq 2000 (Staphylococcus aureus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE203565 |
The Small RNA Teg41 Is a Pleiotropic Regulator of Virulence in Staphylococcus aureus |
|
| Relations |
| BioProject |
PRJNA841379 |
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