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| Status |
Public on May 24, 2022 |
| Title |
Thy1 Marks a Distinct Population of Slow-Cycling Stem Cells in the Mouse Epidermis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we report a discrete population of keratinocytes, marked by Thy1, in the basal layer of the IFE. We find that basal keratinocytes expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than epidermal progenitors and display significant expansion potential in vitro.
Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.
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| Overall design |
We performed gene expression profiling analysis using data obtained from RNA-seq of dorsal epidermal skin of 8-week old mice. 5 different cell populations were isolated using fluorescence-activated cell sorting (FACS). RNA-seq samples were prepared and run in two separate batches, and 3 or more biological replicates were obtained for each sample in total.
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| Contributor(s) |
Fuchs Y, Feldman A, Yusupova M, Gerstberger S |
| Citation(s) |
35941116 |
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| Submission date |
May 19, 2022 |
| Last update date |
Aug 23, 2022 |
| Contact name |
Marianna Yusupova |
| E-mail(s) |
myusupova22@gmail.com
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| Organization name |
Technion- Israel Institute of Technology
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| Street address |
Technion City
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| City |
Haifa |
| ZIP/Postal code |
3200003 |
| Country |
Israel |
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| Platforms (2) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA839761 |
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