 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 18, 2023 |
| Title |
Monocyte - neutrophil interplay drives mesenchymal transition in GBM |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Myeloid cells comprise the majority of immune cells in tumors, where their content and composition is not only driver mutation-specific, but also tumor type-dependent. While these cells are essential for shaping the tumor microenvironment, promoting tumor growth, and contributing to therapeutic resistance, targeting tumor-associated myeloid cells, including bone-marrow-derived monocytes and neutrophils, has not been successful. To eliminate monocyte recruitment, we employed CRISPR/Cas9-based methods to delete the region on murine chromosome 11 harboring the monocyte chemoattractant protein family (MCP) genes, Ccl2, Ccl7, Ccl8, Ccl12, and Ccl11, which we termed qMCP-KO. Using these qMCP-knockout mice in combination with genetically engineered mouse models (GEMM) of glioblastoma (GBM), we investigated myeloid infiltraion by sc-RNA seq. We found that when monocyte infiltraion was abolisehd, a compensatory influx of nuetrophil in these tumor occured. These neutrophil promote tumor growth by releasing TNF, contributing to tumor hypoxic responses and aggression.
|
| |
|
| Overall design |
We induced de novo brain tumor in WT, qMCP-KO mice of both genders. We isolated brain tumor cells at the humane endstage of these mice and ran sc-RNA seq on these cells to compare their functional differences.
|
| |
|
| Contributor(s) |
Hambardzumyan D, Chen Z, Tsankov AM, Giotti B |
| Citation(s) |
37012245 |
| |
| Submission date |
May 17, 2022 |
| Last update date |
May 02, 2023 |
| Contact name |
Dolores Hambardzumyan |
| E-mail(s) |
Dolores.hambardzumyan@mssm.edu
|
| Organization name |
Icahn School of Medicine
|
| Department |
Oncological Sciences
|
| Lab |
Hambardzumyan
|
| Street address |
1425 Madison Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA842512 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203154_RAW.tar |
517.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...