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| Status |
Public on Mar 02, 2023 |
| Title |
CF (1565) vs CFTR-corrected (1564) iPSC_airway at ALI |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Platform: iPSC-derived airway plated on 2D-air liquid interface through basal cell intermediate. Purpose of experiment: To examine the role of mutant CFTR on IPSC-derived airway epithelium (ie. immune dysregulation; dysregulated calcium channel signaling etc). Description of samples:Typical phenotype CF F508del (1565) and syngeneic CFTR-corrected (1564) iPSCs- derived airway epithelium at D90 (D15 CD47hi/CD26lo; replated in 3DMG cultured in 210DCIY x 2 weeks, single-cell passaged and cultured in PNExPlus+dual smad media in 3D, s/p 2 NGFR sorts, cultured at air-liquid interface at 2D)
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| Overall design |
iPSCs- derived airway epithelium from CF and CFTR-corrected syngeneic line, at D90 (D15 CD47hi/CD26lo; replated in 3DMG cultured in 210DCIY x 2 weeks, single-cell passaged and cultured in PNExPlus+dual smad media in 3D, s/p 2 NGFR sorts, cultured at air-liquid interface at 2D)
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| Contributor(s) |
Wang R, Wang F, Lindstrom-Vautrin J, Kotton D |
| Citation(s) |
36857488 |
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| Submission date |
May 13, 2022 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Feiya Wang |
| E-mail(s) |
feiyaw@bu.edu
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| Organization name |
Center for Regenerative Medicine, Boston University
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| Street address |
670 Albany street, 2nd floor
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE203009 |
CF vs CFTR-corrected hiPSC-airway at ALI |
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| Relations |
| BioProject |
PRJNA838010 |
