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| Status |
Public on May 24, 2022 |
| Title |
High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Extremely rare circulating tumor cell (CTC) clusters are both increasingly appreciated as highly metastatic precursors and virtually unexplored. Technologies are primarily designed to detect single CTCs and often fail to account for the fragility of clusters or to leverage cluster-specific markers for higher sensitivity. Meanwhile, the few technologies targeting CTC clusters lack scalability. Here, we introduce the Cluster-Wells, which combines the speed and practicality of membrane filtration with the sensitive and deterministic screening afforded by microfluidic chips. The >100,000 microwells in the Cluster-Wells physically arrest CTC clusters in unprocessed whole blood, gently isolating virtually all clusters at a throughput of >25 mL/h, and allow viable clusters to be retrieved from the device. Using the Cluster-Wells, we isolated CTC clusters ranging from 2–100+ cells from prostate and ovarian cancer patients and analyzed a subset using RNA sequencing. Routine isolation of CTC clusters will democratize research on their utility in managing cancer.
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| Overall design |
Gene expression profiling of circulating tumor cell clusters isolated from two metastatic prostate cancer patients, LNCaP and PC-3 prostate tumor cell lines, and white blood cells of heatlhy donors.
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| Contributor(s) |
Boya M, Ozkaya-Ahmadov T, Swain BE, Chu C, Asmare N, Civelekoglu O, Liu R, Lee D, Tobia S, Biliya S, McDonald LD, Nazha B, Kucuk O, Sanda MG, Benigno BB, Moreno CS, Bilen MA, McDonald JF, Sarioglu AF |
| Citation(s) |
35697674 |
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| Submission date |
May 10, 2022 |
| Last update date |
Jun 27, 2022 |
| Contact name |
A. Fatih Sarioglu |
| E-mail(s) |
sarioglu@gatech.edu
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| Organization name |
Georgia Institute of Technology
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| Department |
Electrical and Computer Engineering
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| Lab |
Sarioglu Lab
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| Street address |
315 Ferst Dr. NW
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30332 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA836891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202650_RAW.tar |
23.7 Mb |
(http)(custom) |
TAR (of TAB, TXT) |
| GSE202650_readcounts_all.csv.gz |
983.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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