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| Status |
Public on Mar 01, 2023 |
| Title |
Transcription profiling of HSCs from SYCNRIP WT and KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SYNCRIP depletion results in HSCs losing their self renewal abilities. Next generation sequencing was conducted in SYNCRIP WT and KO HSCs to investigate the transcriptional changes that occur, and result in the loss of self-renewal.
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| Overall design |
SYNCRIP flox/flox conditional knockout mice were developed for this study. Bone marrow cells from these conditional knockout mice were harvested and transplanted into CD45.1 congenic mice, 6 weeks post transplant mice were intraperitoneally injected with polyinosinic:polycytidylic acid (pIpC). 3 weeks following injections, the transplanted HSCs (CD45.2+, Lin-, Sca1+, cKit+, CD150+, CD48-) were FACS sorted and used for RNA-seq.
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| Contributor(s) |
Vu LP, Herrejon Chavez F, Kharas MG |
| Citation(s) |
37085479 |
| |
| Submission date |
May 08, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Michael Kharas |
| E-mail(s) |
kharasm@mskcc.org
|
| Organization name |
Mamorial Sloan Kettering Cancer Center
|
| Department |
Molecular Pharmacology Program
|
| Lab |
Michael Kharas
|
| Street address |
417 68th street
|
| City |
new york |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA836256 |
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