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| Status |
Public on May 01, 2022 |
| Title |
A Yap-dependent transcriptional program directs cell migration for embryo axis assembly |
| Organism |
Oryzias latipes |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The assembly of the embryo’s primary axis is a fundamental landmark to establish the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. We identified genes involved in cytoskeletal organization and cell-ECM adhesion, rather than in germ layers specification, as direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Yap is acting in migratory cells, in which promotes cortical actin and focal adhesions recruitment. Our results indicate that Yap coordinates a mechano-regulatory loop essential to maintain the directed cell migration sustaining embryo axis development.
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| Overall design |
RNA-seq profile of wild type, yap-/- and yap-/- yap1b-/- mutant embryos at stage 16.
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| Contributor(s) |
Vázquez-Marín J, Sousa-Ortega A, Martínez-Morales JR, Almuedo-Castillo M |
| Citation(s) |
37193708 |
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Ana Sousa-Ortega, Javier Vazquez-Marin, Estefanía Sanabria-Reinoso, Rocío Polvillo, Alejandro Campoy-López, Lorena Buono, Felix Loosli, María Almuedo-Castillo, and Juan R. Martinez-Morales. A Yap-dependent transcriptional program directs cell migration for embryo axis assembly. bioRxiv 2022.02.06.479280; doi:10.1101/2022.02.06.479280
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| Submission date |
Apr 28, 2022 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Javier Vázquez-Marín |
| E-mail(s) |
jvazmar89@gmail.com, javier.vazquez@cos.uni-heidelberg.de
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| Organization name |
Heidelberg University
|
| Department |
Centre for Organismal Studies (COS)
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| Street address |
Im Neuenheimer Feld 230, 5th floor
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| City |
Heidelberg |
| State/province |
Baden-Württemberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL23308 |
Illumina HiSeq 2500 (Oryzias latipes) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA833056 |
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