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| Status |
Public on Feb 12, 2024 |
| Title |
Head to head comparison of target knockdown following OTV vs. anti-TfR:ASO administration in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using single nucleus RNA sequencing, we determined that peripheral administration of OTV:Malat1 results in much more widespread and robust target knockdown throughout the mouse brain compared to anti-TfR:Malat1.
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| Overall design |
TfR.KI mice received four weekly doses of 50mpk OTV:Malat1, anti-TfR:Malat1, Malat1 ASO (at an ASO-equivalent dose), or saline. Tissues were harvested 72 hours after the final dose and snap frozen for molecuar analysis.
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| Web link |
https://www.biorxiv.org/content/10.1101/2023.04.25.538145v1
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| |
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| Contributor(s) |
Barker S, Ha C, Tatarakis D, Sandmann T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 28, 2022 |
| Last update date |
Feb 12, 2024 |
| Contact name |
David Tatarakis |
| E-mail(s) |
tatarakis@dnli.com, genomics@dnli.com
|
| Organization name |
Denali Therapeutics
|
| Department |
Discovery Genomics
|
| Street address |
161 Oyster Point Blvd
|
| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA833038 |
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