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| Status |
Public on Apr 30, 2022 |
| Title |
Effect of NK1.1+ cell depletion on vaginal neutrophil gene expression during HSV-1 infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Depletion of NK1.1+ cells from mice prior to intravaginal HSV-1 infection results in severely increased genital disease and mucosal tissue damage. We determined that this loss of host protection in the absence of NK1.1+ cells was dependent on neutrophils, suggesting that NK1.1+ cells may be modulating neutrophil behavior during infection. To obtain an unbiased view of the transcriptional changes occuring in neutrophils that might explain this difference in disease outcomes, we performed sequencing of total RNA from neutrophils isolated from vaginas of intact or NK1.1 depleted mice 5 days after HSV-1 infection.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for neutrophils sorted from vaginas of control or anti-NK1.1 treated mice 5 days after intravaginal HSV-1 infection.
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| Contributor(s) |
Lee A, Shin H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 27, 2022 |
| Last update date |
May 02, 2022 |
| Contact name |
Haina Shin |
| E-mail(s) |
haina.shin@wustl.edu
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| Organization name |
Washington University School of Medicine
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| Department |
Internal Medicine
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| Street address |
660 South Euclid Ave
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (7)
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| GSM6070029 |
Murine vaginal neutrophils, isotype control biol rep 1, Day 5 |
| GSM6070030 |
Murine vaginal neutrophils, isotype control biol rep 2, Day 5 |
| GSM6070031 |
Murine vaginal neutrophils, isotype control biol rep 3, Day 5 |
| GSM6070032 |
Murine vaginal neutrophils, isotype control biol rep 4, Day 5 |
| GSM6070033 |
Murine vaginal neutrophils, NK1.1 depleted biol rep 1, Day 5 |
| GSM6070034 |
Murine vaginal neutrophils, NK1.1 depleted biol rep 2, Day 5 |
| GSM6070035 |
Murine vaginal neutrophils, NK1.1 depleted biol rep 3, Day 5 |
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| Relations |
| BioProject |
PRJNA832744 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE201713_RAW.tar |
4.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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