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| Status |
Public on Jan 20, 2011 |
| Title |
Systematic meta-analysis and replication of genome-wide expression studies of Parkinson's disease: 3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Systematic meta-analysis and replication of genome-wide expression studies identifies molecular pathways of Parkinson's disease.
Analysis of substantia nigrae from postmortem brains of 6 patients with Parkinson's disease (PD). Results provide insight into the molecular processes perturbed in the PD substantia nigra.
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| Overall design |
Substantia nigra samples from 6 PD and 5 control subjects were obtained. At autopsy, brain hemispheres were frozen in liquid nitrogen and stored at -80C in the Kathleen Price Bryan Brain Bank in the Alzheimer's Disease Research Center at Duke University. Using the RNAgents kit (Promega, Madison, Wis), RNA was extracted from SN and adjacent midbrain tissues. Double-stranded complementary DNA was made with a biotinylated T7(dT)-24 primer. Twenty micrograms of biotinylated complementary RNA was fragmented and hybridized to Affymetrix human genome U133A microarrays. The Affymetrix .CEL files were normalized to "all probe sets" in a standardized matter, and scaled to 100 by the MAS5 algorithm implemented in the Bioconductor package.
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| Contributor(s) |
Hauser MA, Scherzer CR, Zheng B |
| Citation(s) |
20926834 |
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| Submission date |
Feb 02, 2010 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Bin Zheng |
| Organization name |
Brigham and Women's hospital
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| Department |
Center for Neurologic Diseases
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| Street address |
65 Landsdowne Street
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE20186 |
Systematic meta-analysis and replication of genome-wide expression studies of Parkinson's disease |
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| Relations |
| BioProject |
PRJNA129809 |
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