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| Status |
Public on Jun 07, 2022 |
| Title |
Genome-wide chromatin occupancy of MIR205HG/LEADR in prostate basal epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Aside serving as host gene for the microRNA miR-205, MIR205HG transcribes for a nuclear chromatin-associated long noncoding RNA (lncRNA) able to restrain the differentiation of prostate basal cells, a finding that led to its reannotation as LEADR (Long Epithelial Alu-interacting Differentiation-related RNA). In our previous work (PMID: 30659180), we showed that genes that are modulated upon manipulation of MIR205HG/LEADR are characterized by the presence of an Alu sequence in their promoters. Notably, an Alu element also spans the first and second exon of MIR205HG/LEADR, thus suggesting its possible involvement in target selection and binding. Here, we performed Chromatin Isolation by RNA precipitation followed by DNA-sequencing (ChIRP-seq) to map MIR205HG/LEADR chromatin occupancy at a genome-wide level in prostate basal cells.
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| Overall design |
Chromatin Isolation by RNA precipitation followed by DNA-sequencing (ChIRP-seq) for MIR205HG/LEADR long noncoding RNA in RWPE-1 cells, performed in three replicates. LacZ probes used as control.
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| Contributor(s) |
Gandellini P, Bezzecchi E, Pagani G |
| Citation(s) |
35784469 |
| |
| Submission date |
Apr 26, 2022 |
| Last update date |
Sep 09, 2022 |
| Contact name |
Paolo Gandellini |
| E-mail(s) |
paolo.gandellini@unimi.it
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| Phone |
+39 0250314954
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| Organization name |
University of Milan
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| Department |
Bioscience
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| Lab |
Gandellini's lab
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| Street address |
Via celoria 26
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| City |
Milan |
| State/province |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA832237 |
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