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| Status |
Public on Mar 27, 2024 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of Lupus (MRL/lpr) and Control（MRL/mpj）Mouse Microglia Tanscriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Up to 75% of systematic lupus erythematosus (SLE) patients experience neuropsychiatric (NP) symptoms, called neuropsychiatric SLE (NPSLE), yet the underlying mechanisms remain elusive. Microglia control synaptic pruning during early postnatal brain development. The process in NPSLE remains unclear. Here, we show that microglia-coordinated elimination of synaptic terminals participated in NPSLE in MRL/lpr mice, a lupus-prone murine model. We elucidated that lupus mice developed increased depression- and anxiety-like behaviors and persistent phagocytic microglia reactivation before overt peripheral lupus pathology. Microglial engulfment of synapses explained behavioral disorders. To elucidate the mechanism of synaptic pruning by microglia, we sequenced the gene expression in sorted microglia from both lupus (MRL/lpr) mice and the wild-type (MRL/mpj) controls.
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| Overall design |
RNA sequencing in microglia from control(MRL/mpj) and lupus (MRL/lpr) mice, in triplicate.
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| Contributor(s) |
Han X, Sun L |
| Citation(s) |
38494844 |
| |
| Submission date |
Apr 22, 2022 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Han Xiaojuan |
| E-mail(s) |
hxj_719@126.com
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| Phone |
15951950715
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| Organization name |
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
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| Street address |
Zhongshan Road 321
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210008 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA830717 |
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