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| Status |
Public on Jun 01, 2010 |
| Title |
Direct generation of induced pluripotent stem cells from human non-mobilized blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The use of induced pluripotent stem cells (iPSCs) is an exciting frontier in the study and treatment of human diseases through the generation of specific cell types. Here we show the derivation of iPSCs from human non-mobilized peripheral blood (PB) and bone marrow (BM) mononuclear cells (MNCs) by retroviral transduction of OCT3/4, SOX2, KLF4 and c-MYC. The PB- and BM-derived iPSCs were indistinguishable from human embryonic stem cells (hESCs) with respect to morphology, expression of surface antigens and pluripotency-associated transcription factors, global gene expression profiles and differentiation potential in vitro and in vivo. Infected PB and BM MNCs gave rise to iPSCs in the presence of several cytokines, although transduction efficiencies were not high. We found that 5×105 PB MNCs, which corresponds to less than 1 ml of PB, was enough for the generation of several iPSC colonies. Generation of iPSCs from MNCs of non-mobilized PB, with its relative efficiency and ease of harvesting, could enable the therapeutic use of patient-specific pluripotent stem cells.
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| Overall design |
Gene expression profiling was performed in human BMMNCs, PBMNCs, hBMMNC derived iPS cell lines and hPBMNC derived iPS cell lines.
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| Contributor(s) |
Kunisato A, Wakatsuki M, Shinba H, Ota T, Ishida I, Nagao K |
| Citation(s) |
20497033 |
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| Submission date |
Feb 01, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Kenji Nagao |
| E-mail(s) |
kenji.nagao@kyowa-kirin.co.jp
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| Organization name |
Kyowa Hakko Kirin Co., Ltd.
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| Department |
Innovative Drug Research Laboratories
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| Street address |
3-6-6, Asahi-machi,
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| City |
Machida-shi |
| State/province |
Tokyo |
| ZIP/Postal code |
194-8533 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (7)
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| GSM503846 |
human bone marrow mononuclear cells |
| GSM503847 |
human peripheral blood mononuclear cells |
| GSM503848 |
human BMMC derived iPS cells FP#17 |
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| Relations |
| BioProject |
PRJNA124339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20127_RAW.tar |
57.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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