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| Status |
Public on Jun 27, 2022 |
| Title |
IL-6 driven pSTAT1 response is linked to T cell features implicated in early immune dysregulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we performed an in-depth characterization of the effect of classic IL-6 signaling in primary human T cells from healthy individuals. We generated a detailed map of IL-6 signaling in CD4+ and CD8+ naïve and memory T cell subsets, linking mbIL-6R expression levels to the magnitude and balance of phosphorylation of STAT1 and STAT3 and the transcriptomics response to IL-6 in these subsets.
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| Overall design |
Primary human CD3+ T cells derived from healthy donors with the rs2228145 AA genotype were cultured with or without IL-6 (20 ng/mL) for 4 hours and the transcriptomic response to IL-6 signaling was determined in flow sorted T cell subsets including CD4+ naïve (CD4+CD45RA+), CD4+ memory (CD4+CD45RO+) and CD8+ naive (CD8+CD45RA+) T cells.
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| Contributor(s) |
Lambert K, Diggins K, Jones BE, Hundhausen C, Maerz MD, Hocking AM, Sanda S, Greenbaum CJ, Linsley PS, Cerosaletti K, Buckner JH |
| Citation(s) |
35911690 |
| |
| Submission date |
Apr 20, 2022 |
| Last update date |
Apr 23, 2024 |
| Contact name |
Stephanie Osmond |
| E-mail(s) |
sosmond@benaroyaresearch.org
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| Organization name |
Benaroya Research Institute
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| Street address |
1201 9th Ave
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| City |
Seattle, |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (96)
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| Relations |
| BioProject |
PRJNA828611 |
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