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| Status |
Public on Apr 18, 2022 |
| Title |
Real-time quantitative PCR analysis of gene expression in human colon cancer cells treated with LPS |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Relative mRNA levels of 55 genes were evaluated with the SYBR Green qPCR assays after LPS treatment for 8 h.
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| Overall design |
Human colon cancer cells (COLO 225) were purchased from ATCC. The cells in triplicate were treated with various concentrations of LPS for 8 h. RNA isolation and cDNA synthesis were performed as described previously. Relative mRNA levels were evaluated with the SYBR Green qPCR assays using Bio-Rad reagents. BCL2 mRNA was selected as the internal reference for qPCR analysis for this cell type. 1% DMSO treatment was the sample control. The 2-ΔCT and 2-ΔΔCT method of relative quantification was used to determine the fold change in expression.
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| Contributor(s) |
Cao H |
| Citation(s) |
36364387, 37705049, 38504371 |
| |
| Submission date |
Apr 18, 2022 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Heping Cao |
| E-mail(s) |
heping.cao@usda.gov
|
| Phone |
5042864351
|
| Organization name |
USDA-ARS
|
| Department |
Southern Regional Research Center
|
| Street address |
1100 Robert E. Lee Blvd
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70124 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL32173 |
Human mRNA targets analyzed by SYBR Green qPCR |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA827808 |
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