|
| Status |
Public on Jul 18, 2010 |
| Title |
Expression profiling of BRAFV600E melanoma cell lines upon RAF inhibition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray expression analysis to identify global changes in transcription in response to RAF inhibition.
Genes under RAF control were identified in a panel of BRAFV600E tumor cells, following the short-term inhibition of RAF using a pan-RAF kinase inhibitor, PLX4032 (Plexxikon). For comparison with changes in gene expression in response to MEK inhibition using PD0325901 (Pfizer), the following array data was referenced: (http://www.ncbi.nlm.nih.gov/geo/ (accession no. GSE10086)).
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| |
|
| Overall design |
Cell lines growing in culture (n=5) were treated with the RAF inhibitor PLX4032 (250nM or 1000nM) or vehicle alone (0.1% DMSO) as control, for eight hours.
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| |
|
| Contributor(s) |
Joseph EW, Pratilas CA |
| Citation(s) |
20668238 |
| |
| Submission date |
Jan 26, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Christine Anne Pratilas |
| E-mail(s) |
pratilac@mskcc.org
|
| Phone |
646 888 3121
|
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Department |
Molecular Pharmacology
|
| Lab |
Molecular Oncogenesis
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA124317 |
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