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Series GSE20051 Query DataSets for GSE20051
Status Public on Jul 18, 2010
Title Expression profiling of BRAFV600E melanoma cell lines upon RAF inhibition
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Microarray expression analysis to identify global changes in transcription in response to RAF inhibition.
Genes under RAF control were identified in a panel of BRAFV600E tumor cells, following the short-term inhibition of RAF using a pan-RAF kinase inhibitor, PLX4032 (Plexxikon). For comparison with changes in gene expression in response to MEK inhibition using PD0325901 (Pfizer), the following array data was referenced: ( (accession no. GSE10086)).
Overall design Cell lines growing in culture (n=5) were treated with the RAF inhibitor PLX4032 (250nM or 1000nM) or vehicle alone (0.1% DMSO) as control, for eight hours.
Contributor(s) Joseph EW, Pratilas CA
Citation(s) 20668238
Submission date Jan 26, 2010
Last update date Dec 06, 2018
Contact name Christine Anne Pratilas
Phone 646 888 3121
Organization name Memorial Sloan-Kettering Cancer Center
Department Molecular Pharmacology
Lab Molecular Oncogenesis
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM501256 SkMel-1_DMSO
GSM501257 SkMel-1_PLX4032_250
GSM501258 SkMel-5_DMSO
BioProject PRJNA124317

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20051_RAW.tar 28.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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