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| Status |
Public on Oct 23, 2023 |
| Title |
Notch Signaling in the Thyroid is Essential for Adult Homeostasis (RNAseq BAT) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Notch signaling between neighboring cells vitally contributes to specifying their identity and function, during development and in the adult. Here, we demonstrate that selective blockade of the Notch ligands JAG1 and JAG2 disrupts thermoregulation in adults by decreasing circulating thyroid hormones and thyroid function. Sequencing data in BAT demonstrates that Notch inhibition increases thermoregulation genes and this depends on thyroid hormones.
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| Overall design |
Male mice of 10-12 weeks were treated with a single intraperitoneal injection of the Notch blocking antibodies (aJ1aJ2: 20mg/kg each; aN1aN2: 5, 10 mg/kg respectively; aN1aN2aN3: 5,10, 20 mg/kg respectively and anti-Ragweed isotype control: 40 mg/kg). Exogenous administration of thyroxine (T4) and 3-Iodotrionine (T3) was performed by intraperitoneal injection of 1ug/g BW of T4 and 0.05?g/g BW T3 diluted in PBS, daily starting at day 2 of the antibody treatment. Tissues were collected and snap frozen at the time of necropsy.
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| Contributor(s) |
Mosteiro L, Siebel C, Piskol R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 06, 2022 |
| Last update date |
Oct 23, 2023 |
| Contact name |
Robert Piskol |
| E-mail(s) |
piskolr@gene.com
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| Organization name |
Genentech Inc.
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| Department |
Bioinformatics
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| Street address |
1 DNA Way
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (40)
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| This SubSeries is part of SuperSeries: |
| GSE200268 |
Notch Signaling in the Thyroid is Essential for Adult Homeostasis. |
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| Relations |
| BioProject |
PRJNA823911 |
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