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Status |
Public on Dec 22, 2022 |
Title |
A bioengineered tridimensional substrate designed for mesenchymal stem cells culture: whole transcriptomic analysis |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In this work we aimed to evaluate how the three-dimensionality of an engineered scaffold, concieved to emulate the stem cell niche, can influence the whole gene expression profile of mesenchymal stem cells. We performed a differential expression analysis, comparing the transcriptome of MSCs grown in 3D Nichoids with that of MSCs grown on 2D Nichoids, a structure with the same grid geometry, except for the overall tickness which allows a spreading expansion of cells. We found a total of 603 differentially expressed genes that were used also for functional enrichment analysis.
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Overall design |
Total RNA extracted from rBMSCs cultured on 3D Nichoid (n=3) and 2D Nichoid (n=3) after 4 days of culture
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Contributor(s) |
Testa C, Oliveto S, Jacchetti E, Donnaloja F, Martinelli C, Pinoli P, Osellame R, Cerullo G, Ceri S, Biffo S, Raimondi MT |
Citation(s) |
36686258 |
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Submission date |
Mar 31, 2022 |
Last update date |
Feb 06, 2023 |
Contact name |
Carolina Testa |
E-mail(s) |
carolina.testa@polimi.it
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Organization name |
Politecnico di Milano
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Street address |
Piazza Leonardo da Vinci 32
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City |
Milano |
ZIP/Postal code |
20133 |
Country |
Italy |
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Platforms (1) |
GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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Samples (6)
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Relations |
BioProject |
PRJNA821697 |
Supplementary file |
Size |
Download |
File type/resource |
GSE199846_RAW.tar |
760.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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