|
| Status |
Public on Oct 12, 2022 |
| Title |
NURD dependent Reprogramming by Sall4-Jdp2-Esrrb-Glis1 (ATAC-seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Cell fate decision involves rewiring of the genome, but remains poorly understood at the chromatin level. We report a system to reprogramming somatic cells to pluripotency by four factor combination (Sall4, Esrrb, Jdp2, Glis1). Mechanism study demonstrate that the Sall4-NuRD axis plays a critical role in the early phase of reprogramming. These results identify a previously unrecognized role of NuRD in reprogramming, may help establish early chromatin closing as a pre-requisite step in cell fate control.
|
| |
|
| Overall design |
Based on a reprogramming system of 4 factor (WT/N12 Jdp2, Esrrb, Glis1, WT/K5A Sall4), knocking down Gatad2b, Chd4 or dropout single factor.
|
| |
|
| Contributor(s) |
Bo W, Chen L, Jin M |
| Citation(s) |
37208322 |
| |
| Submission date |
Mar 28, 2022 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Li Chen |
| E-mail(s) |
qq809825706@outlook.com
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| State/province |
Guangdong province |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (34)
|
|
| This SubSeries is part of SuperSeries: |
| GSE199614 |
NURD dependent Reprogramming by Sall4-Jdp2-Esrrb-Glis1 |
|
| Relations |
| BioProject |
PRJNA820828 |
Less...
More...