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Series GSE199605 Query DataSets for GSE199605
Status Public on Apr 02, 2022
Title Immune memory after respiratory infection with Streptococcus pneumoniae is revealed by in vitro stimulation of murine splenocytes with inactivated pneumococcal whole cells: evidence of early recall responses by transcriptomic analysis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the present study, we used RNA sequencing on mouse splenocytes to describe the immune recall in the days following pneumococcal lung infection. Mice were sacrificed at days 1, 2, 4, and 7 after Streptococcus pneumoniae (TIGR4 serotype 4) intranasal infection and splenocytes were cultured in the presence or absence of the same inactivated bacterial strain to access the transcriptomic profile.
 
Overall design RNA sequencing on splenocytes of intranasally infected mice sacrificed at 1, 2, 4, or 7 days after infection. Splenocytes were stimulated with inactivated whole pneumococcal cells or not.
 
Contributor(s) Moscardini IF, Santoro F, Carraro M, Gerlini A, Fiorino F, Germoni C, Gholami S, Pettini E, Medaglini D, Iannelli F, Pozzi G
Citation(s) 35795182
Submission date Mar 28, 2022
Last update date Jul 14, 2022
Contact name Francesco Santoro
E-mail(s) santorof@unisi.it
Organization name University of Siena
Department Department of Medical Biotechnologies
Lab Laboratory of Molecular Microbiology and Biotechnology
Street address Viale Bracci, 16, V lotto, I piano
City Siena
State/province SI
ZIP/Postal code 53100
Country Italy
 
Platforms (1)
GPL18635 Ion Torrent Proton (Mus musculus)
Samples (58)
GSM5975983 Unstimulated, uninfected splenocytes mouse 1
GSM5975984 Unstimulated, uninfected splenocytes mouse 4
GSM5975985 Unstimulated, uninfected splenocytes mouse 6
Relations
BioProject PRJNA820830

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Supplementary file Size Download File type/resource
GSE199605_Mouse_counts_20191024.csv.gz 1.5 Mb (ftp)(http) CSV
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