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Series GSE199281 Query DataSets for GSE199281
Status Public on Jul 21, 2022
Title BASEMENT MEMBRANE OF SMALL DIAMETER XENOGENEIC EXTRACELLULAR MATRIX SCAFFOLDS MODULATE QUIESCENT HUMAN ENDOTHELIAL CELL MONOLAYER FORMATION
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Off-the shelf small diameter vascular grafts are an attractive alternative to eliminate the need and shortcomings of autologous tissue for vascular grafting. Bovine saphenous vein (SV) extracellular matrix (ECM) scaffolds from are potentially ideal small diameter vascular grafts, due to their inherit architecture and signaling molecules capable of driving proper cell behavior and regeneration. However, harnessing this potential is predicated on the ability of the scaffold generation technique to maintain the delicate structure, composition and associated function of native vascular ECM. Previous decellularization methods have been uniformly demonstrated to distupt the delicate basement membrane (BM) components of native vascular ECM. Here we demonstrate bovine SV ECM scaffolds generated using the novel antigen removal (AR) approach results in retention of native BM protein composition (e.g., Collagen IV and laminin), structure and cell modulatory function. Presence of BM proteins in AR vascular ECM scaffolds increases endothelial cell migration and proliferation, appropriate formation of adherence junction and apicobasal polarization, increased secretion of nitric oxide, and modulates endothelial cell quiescence. We conclude that presence of intact native vascular BM in AR SV ECM scaffolds modulate human endothelial cell behaviors which are essential for vessel homeostasis.
 
Overall design Gene expression profiling of ECM scaffolds that are treated with TNFa or simvastatin. Sham treated scaffold (i.e. controls) and untreated scaffolds (Scaffolds) were also profiled for gene expression.
 
Contributor(s) Griffiths L, Lopera-Higuita M, Shortreed N, Dasari S
Citation(s) 35983533
Submission date Mar 23, 2022
Last update date Aug 26, 2022
Contact name Surendra Dasari
Organization name Mayo Clinic
Department Quantitative Health Sciences Research
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (24)
GSM5968659 Sham treated scaffold rep 1 [Control1]
GSM5968660 Sham treated scaffold rep 2 [Control2]
GSM5968661 Sham treated scaffold rep 3 [Control3]
Relations
BioProject PRJNA819150

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Supplementary file Size Download File type/resource
GSE199281_merged_gene_count.tsv.gz 2.3 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record
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