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| Status |
Public on Jan 16, 2010 |
| Title |
Expression data from E protein deficient double-positive (DP) thymocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We wanted to test the role of mammalian E proteins E2A and HEB in the development of T cells.
Using a conditional deletion system in which these proteins are deleted at the DP stage of T cell development, we compared DP thymocytes deficient for E2A, HEB or both to wild-type thymocytes
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| Overall design |
We sorted DP thymocytes from wild-type, E2A-deficient, HEB-deficient or E2A/HEB-deficient mice for RNA extraction and hybridization on Affymetrix microarrays. Cells we sorted using fluorescent activated cell sorting and purity was verified as greater that 98%. Approximately 1000000 cells were sorted per sample.
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| Contributor(s) |
D'Cruz LM, Goldrath AW |
| Citation(s) |
20154672 |
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| Submission date |
Jan 15, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Louise D'Cruz |
| Organization name |
UCSD
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM498046 |
wild-type DP cells, biological rep 1 |
| GSM498047 |
wild-type DP cells, biological rep 2 |
| GSM498048 |
wild-type DP cells, biological rep 3 |
| GSM498049 |
E2A-deficient (E2AKO) DP cells, biological rep 1 |
| GSM498050 |
E2A-deficient (E2AKO) DP cells, biological rep 2 |
| GSM498051 |
HEB-deficient (HEBKO) DP cells, biological rep 1 |
| GSM498052 |
HEB-deficient (HEBKO) DP cells, biological rep 2 |
| GSM498053 |
E2A/HEB-deficient (DKO) DP cells, biological rep 1 |
| GSM498054 |
E2A/HEB-deficient (DKO) DP cells, biological rep 2 |
| GSM498055 |
E2A/HEB-deficient (DKO) DP cells, biological rep 3 |
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| Relations |
| BioProject |
PRJNA121993 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19923_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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